An examination of the utility of a nuclear DNA/mitochondrial DNA duplex qPCR assay to assess surface decontamination of hair.
AuthorsDate-Chong M, Buoncristiani MR, Aceves M, Orrego C
JournalForensic Sci Int Genet
PubMed ID23582697
'The goal of this study was to compare two commonly used methods for the surface decontamination of human hair shafts, and to evaluate the use of a duplex real-time qPCR assay to assess decontamination effectiveness for the purpose of mitochondrial DNA typing. Hair shafts of known mitochondrial DNA haplotype were ... More
Contact conductivity detection in poly(methyl methacylate)-based microfluidic devices for analysis of mono- and polyanionic molecules.
Authors Galloway Michelle; Stryjewski Wieslaw; Henry Alyssa; Ford Sean M; Llopis Shawn; McCarley Robin L; Soper Steven A;
JournalAnal Chem
PubMed ID12038768
'An on-column contact conductivity detector was developed for the analysis of various mono- and polyanionic compounds separated by electrophoresis chips fabricated in poly(methyl methacrylate) (PMMA) using hot embossing techniques from Ni electroforms. The detector consisted of a pair of Pt wires (127 microm diameter) with an end-to-end spacing of approximately ... More
MultiPSQ: a software solution for the analysis of diagnostic n-plexed pyrosequencing reactions.
AuthorsDabrowski PW, Schröder K, Nitsche A
JournalPLoS One
PubMed ID23555882
Pyrosequencing can be applied for Single-Nucleotide-Polymorphism (SNP)-based pathogen typing or for providing sequence information of short DNA stretches. However, for some pathogens molecular typing cannot be performed relying on a single SNP or short sequence stretch, necessitating the consideration of several genomic regions. A promising rapid approach is the simultaneous ... More
The use of the MegaBACE for sequencing and genotype analysis.
AuthorsBurger PA
JournalMethods Mol Biol
PubMed ID23546794
Despite the advent of next generation sequencing techniques, which provide access to an enormous amount of genomic information in a relatively short time, the conventional Sanger sequencing and microsatellite genotyping analyses present a straightforward method to answer clearly defined questions in population genetics, phylogeography, or forensics. The MegaBACE is a ... More
The Histone Deacetylase Inhibitor Sodium Butyrate Promotes Cell Death and Differentiation and Reduces Neurosphere Formation in Human Medulloblastoma Cells.
AuthorsNör C, Sassi FA, de Farias CB, Schwartsmann G, Abujamra AL, Lenz G, Brunetto AL, Roesler R
JournalMol Neurobiol
PubMed ID23516101
Increasing evidence suggests that alterations in epigenetic mechanisms regulating chromatin state play a role in the pathogenesis of medulloblastoma (MB), the most common malignant brain tumor of childhood. Histone deacetylase (HDAC) inhibitors, which increase chromatin relaxation, have been shown to display anticancer activities. Here we show that the HDAC inhibitor ... More
Antigenic and genetic characterization of rabies virus isolates from Uruguay.
AuthorsGuarino H, Castilho JG, Souto J, Oliveira Rde N, Carrieri ML, Kotait I
JournalVirus Res
PubMed ID23318595
After 25 years without any reported cases of rabies in Uruguay, the northern region of the country experienced an epizootic of bovine paralytic rabies in October 2007. The outbreak affected bovines and equines, and the main source of infection was the bat Desmodus rotundus, the only hematophagous species in the ... More
Phylogenetic analysis of the mitochondrial genomes and nuclear rRNA genes of ticks reveals a deep phylogenetic structure within the genus Haemaphysalis and further elucidates the polyphyly of the genus Amblyomma with respect to Amblyomma sphenodonti and Amblyomma elaphense.
AuthorsBurger TD, Shao R, Barker SC
JournalTicks Tick Borne Dis
PubMed ID23602360
We sequenced the entire mitochondrial genomes of 3 species of metastriate ticks: Haemaphysalis formosensis, H. parva, and Amblyomma cajennense. We also sequenced two thirds (ca. 9500bp) of the mitochondrial genomes of H. humerosa and H. hystricis. We used these 5 mitochondrial genome sequences together with the 13 tick mitochondrial genomes ... More
Detection of BCR-ABL1 kinase domain mutations causing imatinib resistance in chronic myelogenous leukemia.
AuthorsMoore FR, Yang F, Press RD
Journal
PubMed ID23666688
The reciprocal translocation between chromosomes 9 and 22 [t(9;22)(q34;q11), Philadelphia chromosome] creates a BCR-ABL1 fusion protein that occurs in approximately 95% of cases of chronic myelogenous leukemia (CML), 15% of cases of adult acute lymphoblastic leukemia, and 5% of adult cases of acute myeloid leukemia. The BCR-ABL1 protein is a ... More