La escalera de masa baja de ADN Invitrogen está diseñada para el dimensionamiento y la cuantificación aproximada de ADN bicatenarioMás información
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Número de catálogo
Cantidad
10068013
23,5 μg
Número de catálogo 10068013
Precio (MXN)
-
Cantidad:
23,5 μg
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La escalera de masa baja de ADN Invitrogen está diseñada para el dimensionamiento y la cuantificación aproximada de ADN bicatenario en un intervalo de 100 bp a 2000 bp. La escalera de masa baja de ADN consta de seis fragmentos de ADN individuales purificados mediante cromatografía.
La escalera de masa baja de ADN es idónea para la separación en geles de agarosa al 1–2 %.
Características destacadas de la escalera de masa baja de ADN: • Bandas nítidas y claras: fragmentos purificados mediante cromatografía para ofrecer resultados uniformes y fiables • Cómoda: se suministra con tampón de carga de gel 10X BlueJuice para el seguimiento de la migración de ADN de la muestra • Precisa: una cantidad exacta de ADN en cada banda
Uso del producto La escalera de ADN bicatenaria se puede visualizar en geles de agarosa al 1–2% tras la tinción con bromuro de etidio o SYBR Safe. La escalera está diseñada con bandas de ADN de intensidad creciente. Una cantidad exacta de ADN en cada banda permite la cuantificación aproximada de las muestras de ADN.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración0,1175 μg/μL
Compatibilidad del gelGel de agarosa
Características ecológicasEmbalaje sostenible
N.º de reacciones50 aplicaciones
Tipo de productoMarcador de peso molecular
Cantidad23,5 μg
Listo para cargarNo
Volumen de carga de muestras1 ml
Condiciones de envíoAprobado para su envío a temperatura ambiente o en hielo seco
TecnologíaFragmentos de ADN purificados por cromatografía individual
Volumen (métrico)200 µl
Tipo de gelAgarosa
Intervalo de tamañosDe 100 bp a 2000 bp
Unit SizeEach
Contenido y almacenamiento
• 200 µl Escalera de masa de ADN baja • 1 ml10X BlueJuice Gel Loading Buffer
almacenar en -20° C.
Preguntas frecuentes
Can I know the sequences of Invitrogen DNA ladders?
Sequences of Invitrogen DNA and RNA ladders are proprietary.
Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?
Invitrogen DNA ladders contain linear dsDNA fragments.
Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?
Invitrogen DNA ladders are composed of double-stranded DNA fragments only.
Why are the DNA bands from my molecular weight ladder smearing?
Here are a few reasons why you might see smearing of the bands:
- The DNA was degraded. Avoid nuclease contamination of DNA standards.
- Too much DNA was loaded on the gel. Decrease the amount of DNA in the gel.
- The DNA was contaminated by protein. Remove proteins by phenol extraction before electrophoresis.
- For small DNA, the bands may have diffused during staining. Add the ethidium bromide before electrophoresis.
- For radiolabeled DNA, labeling was performed by nick translation. Label the DNA by replacement synthesis with T4 DNA polymerase or label the 5' end with T4 polynucleotide kinase.
- Improper electrophoresis conditions were used. Do not allow voltage to exceed ~20 V/cm. Maintain a temperature <30°C during electrophoresis. Check that the electrophoresis buffer used has sufficient buffering capacity.
- The DNA contained too much salt. Remove excess salt by ethanol precipitation before electrophoresis.
I'm seeing anomalous migration of my DNA ladder. What happened?
This can happen if the marker was heated. Please ensure that the ladders are not heated before use.
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