Low DNA Mass Ladder This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 10068013

Invitrogen™  Related applications: Nucleic Acid Gel Electrophoresis & Blotting

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Description

The Low DNA Mass Ladder is suitable for estimating the mass (quantity) of unknown DNA samples by ethidium bromide staining. Prepared from a specially constructed plasmid, the ladder consists of an equimolar mixture of six blunt-ended fragments from 100 to 2,000 bp. Electrophoresis of 4 µL of Low DNA Mass Ladder results in bands containing 200, 120, 80, 40, 20, and 10 ng (470 ng total) of DNA, respectively.

Product use
For reliable comparison of band intensities, the loading volume of the ladder should be the same as the volume of the experimental sample. Smaller volumes routinely give sharper bands. Do not heat the ladder before loading.

Note: The closer the size of the sample band relative to the band of comparable intensity in the Low DNA Mass Ladder, the more accurate the mass estimation will be.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Compatibility: Agarose Gels
Ready to Load: No
Mass: 23.5 µg
Size Range: 0.1 to 2 kb
Product Size: 50 applications
Concentration: 117.5 ng⁄µl
Volume (Metric): 200 µl
Number of Reactions: 50 Applications
Green Features: Less waste, Sustainable packaging
Shipping Condition: Approved for shipment at Room Temperature or on Dry Ice

Contents & storage

Contains: Low DNA Mass™ Ladder, 200 µL in 10 mM Tris-HCl (pH 7.5), 1 mM EDTA.

Store at -20°C.

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