ChIP-Seq using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites.
AuthorsLefrançois P, Zheng W, Snyder M
JournalMethods Enzymol
PubMed ID20946807
'Much of eukaryotic gene regulation is mediated by binding of transcription factors near or within their target genes. Transcription factor binding sites (TFBS) are often identified globally using chromatin immunoprecipitation (ChIP) in which specific protein-DNA interactions are isolated using an antibody against the factor of interest. Coupling ChIP with high-throughput ... More
Post intrastromal corneal ring segments insertion complicated by Candida parapsilosis keratitis.
AuthorsMitchell BM, Kanellopoulos AJ, Font RL
JournalClin Ophthalmol
PubMed ID23467516
'This case report describes the clinical and histopathologic features, including molecular confirmation, of fungal keratitis after intrastromal corneal ring segments placement for keratoconus. A 52-year-old woman underwent insertion of Intacs(®) corneal implants for treatment of keratoconus. Extrusion of the implants was noted 5 months post insertion and replaced. Three months ... More
Development of PCR-RFLP assay for the discrimination of Plasmodium species and variants of P. vivax (VK210, VK247 and P. vivax-like) in Anopheles mosquitoes.
'The identification of Plasmodium species in Anopheles mosquitoes is an integral component of malaria control programs. We developed a new assay to identify Plasmodium falciparum, Plasmodium malariae, and Plasmodium vivax variants. Specific primers were designed to hybridize to CS gene-specific regions. Polymerase chain reaction (PCR) and restriction fragment length polymorphism ... More
Report of an international collaborative study to evaluate the suitability of multiplex PCR as an identity assay for different sub-strains of BCG vaccine.
AuthorsMarkey K, Ho MM, Choudhury B, Seki M, Ju L, Castello-Branco LR, Gairola S, Zhao A, Shibayama K, Andre M, Corbel MJ
JournalVaccine
PubMed ID20732463
'Current methods for the identification of BCG vaccine in quality control settings involve acid-fast staining with microscopic examination. However, this method is unable to distinguish the many different sub-strains of BCG, or to differentiate BCG strains from virulent members of the Mycobacterium tuberculosis complex. A multiplex PCR (mPCR) which uses ... More
Identification of novel mutation in cathepsin C gene causing Papillon-Lefèvre Syndrome in Mexican patients.
AuthorsRomero-Quintana JG, Frías-Castro LO, Arámbula-Meraz E, Aguilar-Medina M, Dueñas-Arias JE, Melchor-Soto JD, Romero-Navarro JG, Ramos-Payán R
JournalBMC Med Genet
PubMed ID23311634
Papillon-Lefèvre Syndrome (PLS) is a type IV genodermatosis caused by mutations in cathepsin C (CTSC), with a worldwide prevalence of 1-4 cases per million in the general population. In México, the prevalence of this syndrome is unknown, and there are few case reports. The diagnosis of twenty patients in the ... More
Cost-effective and scalable DNA extraction method from dried blood spots.
AuthorsSaavedra-Matiz CA, Isabelle JT, Biski CK, Duva SJ, Sweeney ML, Parker AL, Young AJ, Diantonio LL, Krein LM, Nichols MJ, Caggana M
JournalClin Chem
PubMed ID23509109
Dried blood spot (DBS) samples have been widely used in newborn screening (NBS) for the early identification of disease to facilitate the presymptomatic treatment of congenital diseases in newborns. As molecular genetics knowledge and technology progresses, there is an increased demand on NBS programs for molecular testing and a need ... More
In vitro anaerobic biofilms of human colonic microbiota.
AuthorsSproule-Willoughby KM, Stanton MM, Rioux KP, McKay DM, Buret AG, Ceri H
JournalJ Microbiol Methods
PubMed ID20920538
The human gastrointestinal tract hosts a complex community of microorganisms that grow as biofilms on the intestinal mucosa. These bacterial communities are not well characterized, although they are known to play an important role in human health. This study aimed to develop a model for culturing biofilms (surface-adherent communities) of ... More
HPV-18 E6 mutants reveal p53 modulation of viral DNA amplification in organotypic cultures.
AuthorsKho EY, Wang HK, Banerjee NS, Broker TR, Chow LT,
JournalProc Natl Acad Sci U S A
PubMed ID23572574
Human papillomaviruses (HPVs) amplify in differentiated strata of a squamous epithelium. The HPV E7 protein destabilizes the p130/retinoblastoma susceptibility protein family of tumor suppressors and reactivates S-phase reentry, thereby facilitating viral DNA amplification. The high-risk HPV E6 protein destabilizes the p53 tumor suppressor and many other host proteins. However, the ... More
An investigation of the presence of specific anaerobic species in necrotic primary teeth.
AuthorsGomes GB, Sarkis-Onofre R, Bonow ML, Etges A, Jacinto RC
JournalBraz Oral Res
PubMed ID23538426
Different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. The aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (PCs) and root canals (RCs) of infected primary teeth using PCR methods. Paired PC ... More
Multidisciplinary evidences that Synechocystis PCC6803 exopolysaccharides operate in cell sedimentation and protection against salt and metal stresses.
AuthorsJittawuttipoka T, Planchon M, Spalla O, Benzerara K, Guyot F, Cassier-Chauvat C, Chauvat F
JournalPLoS One
PubMed ID23405172
Little is known about the production of exopolysaccharides (EPS) in cyanobacteria, and there are no genetic and physiological evidences that EPS are involved in cell protection against the frequently encountered environmental stresses caused by salt and metals. We studied four presumptive EPS production genes, sll0923, sll1581, slr1875 and sll5052, in ... More