Search

  • 訂單查詢
  • 快速訂購

RNaseOUT™ Recombinant Ribonuclease Inhibitor - FAQs

View additional product information for RNaseOUT™ Recombinant Ribonuclease Inhibitor - FAQs (10777019)

6 product FAQs found

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

What does RNaseOUT Recombinant RNase Inhibitor do?

The inhibitor acts as a safeguard against degradation of target RNA due to ribonuclease contamination of the RNA preparation.

What is the concentration of RNaseOUT Recombinant Ribonuclease Inhibitor in Units/microL?

RNaseOUT Recombinant Ribonuclease Inhibitor is provided at a concentration of 40 U/µL.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the temperature limitation of RNaseOUT Recombinant Ribonuclease Inhibitor?

RNaseOUT Recombinant Ribonuclease Inhibitor maintains full activity from 4 degrees C to 55 degrees C. Activity decreases at higher temperatures, and heating to 65 degrees C inactivates the enzyme. Under prolonged reaction conditions, the inhibitor remains active for at least 96 hours at 4 degrees C and 25 degrees C, 24 hours at 37 degrees C, and up to 6 hours at 50 degrees C.

I am having trouble performing PCR amplification after DNA isolation with RNAlater-treated samples. Does RNAlater have an impact on downstream PCR applications?

RNAlater should not impact downstream PCR amplification as long as the sample has been properly cleaned before proceeding with DNA isolation, as stated in the RNAlater Stabilization Solution manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/7020M.pdf) on page 7.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

What is the difference between RNAlater Stabilization Solution and RNaseOUT Recombinant Ribonuclease Inhibitor? When do I use one over the other?

RNAlater Stabilization Solution stabilizes and protects cellular RNA in intact, unfrozen tissue samples, eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. In other words, it is an RNA preserving reagent.

RNaseOUT Recombinant Ribonuclease Inhibitor is a potent non-competitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications such as cDNA synthesis, RT-PCR, and in vitro transcription and translation. RNAseOUT inhibits RNase A, RNAse B, and RNAse C. In other words, RNaseOUT Recombinant Ribonuclease Inhibitor is mainly used in an assay to prevent RNA degradation.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.