'Progenitor and pluripotent cell types offer promise as regenerative therapies but transfecting these sensitive cells has proven difficult. Herein, a series of linear trehalose-oligoethyleneamine "click" copolymers were synthesized and examined for their ability to deliver plasmid DNA (pDNA) to two progenitor cell types, human dermal fibroblasts (HDFn) and rat mesenchymal ... More
Comparison of conventional PCR, quantitative PCR, bacteriological culture and the Warthin Starry technique to detect Leptospira spp. in kidney and liver samples from naturally infected sheep from Brazil.
AuthorsFornazari F, da Silva RC, Richini-Pereira VB, Beserra HE, Luvizotto MC, Langoni H
JournalJ Microbiol Methods
PubMed ID22713608
'Leptospirosis is an infectious disease of worldwide importance. The development of diagnostic techniques allows sick animals to be identified, reservoirs to be eliminated and the disease prevented and controlled. The present study aimed to compare different techniques for diagnosing leptospirosis in sheep. Samples of kidney, liver and blood were collected ... More
Periodontopathogens around the surface of mini-implants removed from orthodontic patients.
AuthorsTortamano A, Dominguez GC, Haddad AC, Nunes FD, Nacao M, Morea C
JournalAngle Orthod
PubMed ID22839769
To verify if mini-implant mobility is affected by the presence of periodontopathogens, frequently associated with peri-implantitis.
Microarray-CGH for the assessment of aneuploidy in human polar bodies and oocytes.
AuthorsJaroudi S, Wells D
JournalMethods Mol Biol
PubMed ID23138959
The cytogenetic analysis of single cells, such as oocytes and polar bodies, is extremely challenging. The main problem is low probability of obtaining a metaphase preparation in which all of the chromosomes are sufficiently well spread to permit accurate analysis (no overlapping chromosomes, no chromosomes lost). As a result, a ... More
Cloning of cytokine 3' untranslated regions and posttranscriptional assessment using cell-based GFP assay.
AuthorsAl-Haj L, Khabar KS
JournalMethods Mol Biol
PubMed ID22131027
Cytokine biosynthesis is tightly regulated by a number of processes, including gene expression control. Posttranscriptional control of cytokine gene expression offers a fine-tuning mechanism that contributes not only to transient biosynthesis of cytokines, but also helps in rapid and early initiation of the cytokine response. Deregulation of cytokine biosynthesis has ... More
Mouse gastrocnemius muscle regeneration after mechanical or cardiotoxin injury.
AuthorsCzerwinska AM, Streminska W, Ciemerych MA, Grabowska I
JournalFolia Histochem Cytobiol
PubMed ID22532150
The goal of our study was to compare the skeletal muscle regeneration induced by two types of injury: either crushing, that causes muscle degeneration as a result of mechanical devastation of myofibers, or the injection of a cardiotoxin that is a myotoxic agent causing myolysis of myofibers leading to muscle ... More
Construction and utilization of carotenoid reporter systems: identification of chromosomal integration sites that support suitable expression of biosynthetic genes and pathways.
AuthorsSharpe PL, DiCosimo DJ
JournalMethods Mol Biol
PubMed ID22623306
In order to metabolically engineer microorganisms to produce compounds of interest, it is often desirable to integrate foreign genes into the chromosome of the host. However, the consequences of these genetic alterations are not always predictable. The use of a reporter system can often assist in determining chromosomal locations for ... More
Engineering Escherichia coli for canthaxanthin and astaxanthin biosynthesis.
AuthorsCheng Q, Tao L
JournalMethods Mol Biol
PubMed ID22623300
Escherichia coli is a non-carotenogenic bacterium that could synthesize farnesyl pyrophosphate precursor through the isoprenoid pathway. Carotenoid production in E. coli requires heterologous expression of carotenoid synthesis genes. The carotenoid synthesis operons are assembled from genes isolated from carotenogenic bacterial sources. Expression of the different operons yields different carotenoid titers. ... More
First identification of Ewing's sarcoma-derived extracellular vesicles and exploration of their biological and potential diagnostic implications.
AuthorsMiller IV, Raposo G, Welsch U, Prazeres da Costa O, Thiel U, Lebar M, Maurer M, Bender HU, von Luettichau I, Richter GH, Burdach S, Grunewald TG
JournalBiol Cell
PubMed ID23521563
Exosomes are small RNA- and protein-containing extracellular vesicles (EVs) that are thought to mediate hetero- and homotypic intercellular communication between normal and malignant cells. Tumour-derived exosomes are believed to promote re-programming of the tumour-associated stroma to favour tumour growth and metastasis. Currently, exosomes have been intensively studied in carcinomas. However, ... More
Microbial diversity differences within aerobic granular sludge and activated sludge flocs.
AuthorsWinkler MK, Kleerebezem R, de Bruin LM, Verheijen PJ, Abbas B, Habermacher J, van Loosdrecht MC
JournalAppl Microbiol Biotechnol
PubMed ID23064482
In this study, we investigated during 400 days the microbial community variations as observed from 16S DNA gene DGGE banding patterns from an aerobic granular sludge pilot plant as well as the from a full-scale activated sludge treatment plant in Epe, the Netherlands. Both plants obtained the same wastewater and had ... More
Detection of the full-length transcript variant for neurokinin-1 receptor in human whole blood associated with enhanced reinforcement of clot by substance-P.
AuthorsAzma T, Sugimoto Y, Kinoshita H, Ito T, Tsukamoto M, Hoshijima H, Nakao M, Kikuchi H
JournalJ Thromb Thrombolysis
PubMed ID22057425
We have recently reported that a neurotransmitter for pain, substance-P (SP), promotes platelet-dependent clot formation through neurokinin-1 receptors (NK1Rs), in which leukocytes appear to be involved (J Thromb Thrombolysis 2009;27:280-6). Two naturally occurring splice isoforms of NK1R with different signal transduction potency, namely the full-length and the truncated NK1Rs are ... More