Platinum® Taq DNA Polymerase

Catalog number: 10966018

Invitrogen™  Related applications: Chromatin Biology | Methylation Analysis | PCR

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Platinum® Taq DNA Polymerase is a convenient and reliable "hot start" thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The "hot start" property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step. Features of Platinum® Taq DNA Polymerase include:

Convenience—assemble PCR reactions at room temperature
Specificity—"hot start" kinetics reduce nonspecific primer annealing, improving product yield
Product size—use for PCR products up to 10 kb
Flexibility—optional KB Extender gives more versatility in PCR assays

Using Platinum® Taq DNA Polymerase
Just as with Taq DNA Polymerase, Platinum® Taq DNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity (but not 3′→5′ exonuclease activity). PCR products generated with Platinum® Taq DNA Polymerase may be used in the same downstream applications without protocol modifications. Use Platinum® Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard, Fast
Final Product Size(s): 5 kb or less
Product Size: 120 reactions
Exonuclease Activity: 5' - 3'
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Each box contains:
• 24 µl Platinum® Taq DNA Polymerase
• 1.25 mL 10X PCR Buffer (no magnesium chloride)
• 1 mL 50 mM magnesium chloride
• 1.3 mL KB Extender

Store at -30°C to -10°C in a non-frost-free freezer.

Documents

Manuals & protocols

Recommended products