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Invitrogen

IL-17AF Monoclonal Antibody (20LJS09), FITC, eBioscience™

View all (7) IL-17AF antibodies

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Product Details

11-9179-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

20LJS09

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149871

Product Specific Information

Description: The 20LJS09 monoclonal antibody reacts with human interleukin (IL)-17AF heterodimer. This antibody specifically reacts with this heterodimer and has no reactivity for IL-17A or IL-17F homodimers. IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.

Applications Reported: This 20LJS09 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 20LJS09 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of in vitro-polarized human Th17 cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: CTLA-8; cytokine; Cytokine ML-1; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; IL-17F; ILN; inflammatory cytokine; Interleukin; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin-17; Interleukin-17A; Interleukin-17F

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Gene Aliases: CANDF6; CTLA-8; CTLA8; IL-17; IL-17A; IL-17F; IL17; IL17A; IL17F; ILA17; ML-1; ML1

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UniProt ID: (Human) Q16552, (Human) Q96PD4

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Entrez Gene ID: (Human) 3605, (Human) 112744

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Function(s)
cytokine activity protein binding protein homodimerization activity protein heterodimerization activity cytokine receptor binding cytokine binding
Process(es)
positive regulation of antimicrobial peptide production adaptive immune response immune system process apoptotic process inflammatory response immune response Notch signaling pathway cell-cell signaling cell death response to wounding gene expression positive regulation of keratinocyte proliferation keratinocyte differentiation positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-16 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production interleukin-17A-mediated signaling pathway keratinocyte proliferation innate immune response positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter defense response to Gram-negative bacterium defense response to Gram-positive bacterium defense response to fungus positive regulation of protein kinase B signaling intestinal epithelial structure maintenance cellular response to interleukin-1 fibroblast activation regulation of primary metabolic process interleukin-17-mediated signaling pathway granulocyte migration negative regulation of inflammatory response to wounding positive regulation of cytokine production involved in inflammatory response positive regulation of bicellular tight junction assembly positive regulation of tumor necrosis factor superfamily cytokine production negative regulation of interleukin-17-mediated signaling pathway positive regulation of chemokine (C-X-C motif) ligand 1 production positive regulation of cytokine production negative regulation of angiogenesis regulation of transforming growth factor beta receptor signaling pathway regulation of granulocyte macrophage colony-stimulating factor production regulation of interleukin-2 production regulation of interleukin-6 production regulation of interleukin-8 production positive regulation of lymphotoxin A production cartilage development
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