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Platinum® Taq DNA Polymerase High Fidelity

Catalog number: 11304011

Invitrogen™  Related applications: Chromatin Biology | Methylation Analysis | PCR | Sanger Sequencing

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Description

Platinum® Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. Features of Platinum® Taq DNA Polymerase, High Fidelity:

Fidelity—greater than six times higher fidelity than Taq DNA polymerase
Amplicon size—amplification of fragments up to 15 kb (see figure)
Convenience—room temperature reaction assembly
Applications—amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR

Unit definition
One unit of Platinum® Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 6 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: Mixed
Reaction Speed: Standard
Final Product Size(s): 20 kb or less
Exonuclease Activity: 3' - 5', 5' - 3'
Product Size: 100 reactions
Shipping Condition: Dry Ice

Contents & storage

Platinum® Taq DNA Polymerase High Fidelity (20 µL, at 5U/µL) is supplied with a vial of 10X High Fidelity Buffer [600 mM Tris-SO4 (pH 8.9), 180 mM (NH4)2SO4] (1.25 mL), and a vial of 50 mM MgSO4 (1 mL).

Store at -30°C to -10°C.

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Manuals & protocols

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