Grace's Insect Medium, supplemented This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  11605094

 Related applications:

Insect Cell Culture

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Grace's Insect Medium, Supplemented, was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21.

Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco® media options for a range of insect cell culture applications.

This Grace's Insect Medium contains lactalbumin hydrolysate, yeastolate, L-glutamine, and sodium bicarbonate. The complete formulation is available. Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.

Dual site cGMP manufacturing and quality system
Gibco® Grace's Supplemented Insect Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical product made in our Scotland facility (11605-045). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate, and is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. Grace's Supplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.


Cell Line: Sf21, Sf9
Cell Type: Insect Cells
Form: Liquid
Glutamine: L-Glutamine
Inorganic Salts: Calcium, Magnesium
Product Line: Gibco®
Product Size: 500 mL
Supplements: Insect Hemolymph-Free, Lactalbumin Hydrolysate, Yeastolate
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture


Manuals & protocols