Bombyxin is a growth factor for wing imaginal disks in Lepidoptera.
Authors Nijhout H Frederik; Grunert Laura W;
JournalProc Natl Acad Sci U S A
PubMed ID12429853
'The mechanisms that control the growth rate of internal tissues during postembryonic development are poorly understood. In insects, the growth rate of imaginal disks varies with nutrition and keeps pace with variation in somatic growth. We describe here a mechanism by which the growth of wing imaginal disks is controlled. ... More
Kinesin Superfamily Motor Protein KIF17 and mLin-10 in NMDA Receptor-Containing Vesicle Transport
'Experiments with vesicles containing N-methyl-D-aspartate (NMDA)receptor 2B (NR2B subunit) show that they are transported alongmicrotubules by KIF17, a neuron-specific molecular motor in neuronaldendrites. Selective transport is accomplished by direct interaction of theKIF17 tail with a PDZ domain of mLin-10 (Mint1/X11), which is aconstituent of a large protein complex including mLin-2 ... More
Interaction of nucleotides with membrane-associated cystic fibrosis transmembrane conductance regulator.
AuthorsTravis SM, Carson MR, Ries DR, Welsh MJ
JournalJ Biol Chem
PubMed ID7687995
'Cystic fibrosis transmembrane conductance regulator (CFTR) is a Cl- channel that is regulated by cytosolic nucleotides and by cAMP- dependent phosphorylation. In excised membrane patches, CFTR Cl- channel activity requires hydrolyzable nucleotides and Mg2+, and is inhibited by ADP. We examined the interactions between CFTR and nucleotides using 8-azidoadenosine 5''-triphosphate ... More
Purification, reconstitution, and steady-state kinetics of the trans-membrane 17 beta-hydroxysteroid dehydrogenase 2.
Authors Lu Ming-Liang; Huang Yi-Wei; Lin Sheng-Xiang;
JournalJ Biol Chem
PubMed ID11940569
'Human membrane 17 beta-hydroxysteroid dehydrogenase 2 is an enzyme essential in the conversion of the highly active 17beta-hydroxysteroids into their inactive keto forms in a variety of tissues. 17 beta-hydroxysteroid dehydrogenase 2 with 6 consecutive histidines at its N terminus was expressed in Sf9 insect cells. This recombinant protein retained ... More
Estimation of baculovirus titer by beta-galactosidase activity assay of virus preparations.
AuthorsYahata T, Andriole S, Isselbacher KJ, Shioda T
JournalBiotechniques
PubMed ID10948416
There is no abstract available. The title describes the paper.
Functional chicken gizzard heavy meromyosin expression in and purification from baculovirus-infected insect cells [published erratum appears in Proc Natl Acad Sci U S A 1995 Mar 28;92(7):3076]
AuthorsOnishi H, Maeda K, Maeda Y, Inoue A, Fujiwara K
JournalProc Natl Acad Sci U S A
PubMed ID7846039
The heavy chain and the essential and the regulatory light chains of chicken gizzard heavy meromyosin (HMM) were coexpressed in Spodoptera frugiperda (fall armyworm) cells infected with a mixture of two recombinant Autographa californica baculoviruses. Soluble HMM consisting of the heavy chain and the two types of light chains was ... More
A novel 43-kDa protein as a negative regulatory component of phenoloxidase-induced melanin synthesis.
AuthorsZhao M, Söderhäll I, Park JW, Ma YG, Osaki T, Ha NC, Wu CF, Söderhäll K, Lee BL,
JournalJ Biol Chem
PubMed ID15857824
The melanization reaction induced by activated phenoloxidase in arthropods is important in the multiple host defense innate immune reactions, leading to the sequestration and killing of invading microorganisms. This reaction ought to be tightly controlled because excessive formation of quinones and systemic hypermelanization are deleterious to the hosts, suggesting that ... More
Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor.
Authors Liedtke Carole M; Yun C H Chris; Kyle Nicole; Wang Dandan;
JournalJ Biol Chem
PubMed ID11956211
Protein kinase C (PKC) regulation of cystic fibrosis transmembrane regulator (CFTR) chloride function has been demonstrated in several cell lines, including Calu-3 cells that express native, wild-type CFTR. We demonstrated previously that PKC epsilon was required for cAMP-dependent CFTR function. The goal of this study was to determine whether PKC ... More
Hedgehog-stimulated Phosphorylation of the Kinesin-related Protein Costal2 Is Mediated by the Serine/Threonine Kinase Fused.
Authors Nybakken Kent E; Turck Christoph W; Robbins David J; Bishop J Michael;
JournalJ Biol Chem
PubMed ID11934882
The Hedgehog (Hh) signaling molecule is required for the development of numerous tissues in Drosophila. Within the cell, Hh signal transduction utilizes a large protein complex consisting of the Fused (Fu), Costal2 (Cos2), and Cubitis interruptus (Ci) proteins, but the functional interactions between these proteins are still largely uncharacterized. Using ... More