Analysis of one-step and two-step real-time RT-PCR using SuperScript III.
AuthorsWacker MJ,Godard MP
JournalJournal of biomolecular techniques : JBT
PubMed ID16461951
Real-time reverse transcription polymerase chain reaction (RT-PCR) is a commonly used technique to analyze gene expression. There has been little research conducted to test if SuperScript III quantitative one-step (reverse transcription carried out in the same tube as PCR) and two-step (reverse transcription carried out in a separate reaction) RT-PCR ... More