SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX
Product Image
Citations & References (1)
Invitrogen™

SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX

The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient詳細を見る
テクニカルサポートオーダーサポート
表示を変更buttonViewtableView
製品番号（カタログ番号）反応数
11745500500 Reactions
11745100反応100回分
製品番号（カタログ番号） 11745500
価格（JPY）
333,100
Each
お問い合わせください ›
反応数:
500 Reactions
The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient one-step format and consistent performance make it ideal for a number of gene expression applications, including infectious disease surveillance. The kit has been validated by several global public health organizations and has long been the choice of the US CDC for infectious disease surveillance.

Features of the SuperScript III Platinum One-Step qRT-PCR Kit include:
• SuperScript III RT for higher temperature cDNA synthesis when addressing difficult RNA secondary structure
• Platinum Taq DNA Polymerase with hot-start technology for improved specificity
• LUX fluorogenic primers and dual-labeled fluorogenic probes for effective detection performance
• ROX reference dye pre-mixed at optimal concentration for Applied Biosystems instruments
• Validated performance on multiple real-time instrument platforms, including rotor-based systems

SuperScript III Platinum One-Step qRT-PCR Kits come in multiple configurations:
SuperScript III Platinum One-Step qRT-PCR Kit with ROX passive reference (this page)
SuperScript III Platinum One-Step qRT-PCR Kit
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit with ROX passive reference

研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7000 System, 7300 System, 7700 System, 7900HT System
反応数500 Reactions
ポリメラーゼPlatinum Taq DNA Polymerase
製品ラインPlatinum, SuperScript
製品タイプOne-Step qRT-PCR Kit
数量500 reactions
サンプルタイプRNA
出荷条件ドライアイス
対応可能対象500 Reactions
検出法Primer-probe
使用対象（アプリケーション）遺伝子発現解析, Pathogen Detection, Virus Detection
PCR法1-step RT-qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
• 500 μL SuperScript™ III/Platinum™ Taq Mix
• 12.5 mL 2X Reaction Mix with ROX
• 2 × 1 mL Magnesium Sulfate (50 mM)

Store components in the dark at -20°C.

よくあるご質問（FAQ）

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX, 500 reactions FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Analysis of one-step and two-step real-time RT-PCR using SuperScript III.
Authors:Wacker MJ,Godard MP
Journal:Journal of biomolecular techniques : JBT
PubMed ID:16461951
Real-time reverse transcription polymerase chain reaction (RT-PCR) is a commonly used technique to analyze gene expression. There has been little research conducted to test if SuperScript III quantitative one-step (reverse transcription carried out in the same tube as PCR) and two-step (reverse transcription carried out in a separate reaction) RT-PCR ... More