Invitrogen™

SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Catalog number:  11732088

Related applications:

Real Time PCR-Based Gene Expression Profiling

| Real Time PCR (qPCR)

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Description

The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient one-step format and consistent performance make it ideal for a number of gene expression applications, including infectious disease surveillance. The kit has been validated by several global public health organizations and has long been the choice of the US CDC for infectious disease surveillance.

Features of the SuperScript III Platinum One-Step qRT-PCR Kit include:
• SuperScript III RT for higher temperature cDNA synthesis when addressing difficult RNA secondary structure
• Platinum Taq DNA Polymerase with hot-start technology for improved specificity
• LUX fluorogenic primers and dual-labeled fluorogenic probes for effective detection performance
• Validated performance on multiple real-time instrument platforms, including rotor-based systems

SuperScript III Platinum One-Step qRT-PCR Kits come in multiple configurations:
SuperScript III Platinum One-Step qRT-PCR Kit (this page)
SuperScript III Platinum One-Step qRT-PCR Kit with ROX passive reference
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit with ROX passive reference

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Product Line: Platinum®, SuperScript™
Sample Type: RNA
Shipping Condition: Dry Ice
Detection Method: Primer-Probe Detection
PCR Method: 1-Step qRT-PCR
Reaction Speed: Standard
Sufficient For: 500 Reactions

Contents & storage

• 500 µl SuperScript® III/Platinum® Taq Mix
• 12.5 ml 2X Reaction Mix
• 2 × 1 ml Magnesium Sulfate (50 mM)
• 500 µl ROX Reference Dye (25 µM)

Store components at -20°C. ROX Reference Dye must be stored in the dark.