EXPRESS One-Step Superscript™ qRT-PCR Kit, universal

Catalog number:  1178101K

 Related applications:

Real Time PCR (qPCR)

| Real Time PCR-Based Gene Expression Profiling

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EXPRESS One-Step SuperScript® qRT-PCR SuperMix Kits incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments.

These kits provide:

Fast-activating, antibody-mediated Platinum® Taq DNA Polymerase—complete, rapid activation provides the best sensitivity and reproducibility
The One-Step SuperScript® III Reverse Transcriptase module—a unique formulation to ensure better yield and less PCR inhibition, specifically for one-step qRT-PCR
Flexible formats with ROX premixed or separate—designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler® 480, Corbett Rotor-Gene™)
The only commercially available one-step qRT-PCR kits containing heat-labile UDG—significantly reduced carryover contamination risk with excellent activity from room temperature to approximately 40°C. Due to the thermostability of SuperScript® III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated

Easy to test and switch
EXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient and stable SuperMix format for ultimate convenience. The kits are offered with either premixed ROX or a separate tube of ROX to fit your instrument needs.
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step qRT-PCR
Detection Method: Primer-Probe Detection
Reaction Speed: Fast, Standard
For Use With (Equipment): 7500 Fast System, 7500 System, BioRad CFX96™, BioRad MyiQ, BioRad iCycler iQ, Cepheid SmartCycler, Corbett RotorGene, MJ Chromo4, MJ Opticon, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type (General): RNA
Product Line: SuperScript™
Product Size: 2,500 reactions

Contents & storage

The EXPRESS One-Step SuperScript® qRT-PCR Universal kit contains 3 modules: 2X EXPRESS SuperScript® Mix (5 × 1 mL), Express qPCR SuperMix Universal (5 × 5 mL), and ROX Reference Dye (5 × 500 µL).

Store at -20°C for long-term storage. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4–8°C.


Manuals & protocols