EXPRESS One-Step SYBR™ GreenER™ Kit, universal
Product Image
Invitrogen™

EXPRESS One-Step SYBR™ GreenER™ Kit, universal

EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments. This kit comes with a자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호충분
1178001K
11780-01K으로도 사용됨
2500 Reactions
11780200500 Reactions
카탈로그 번호 1178001K
11780-01K으로도 사용됨
제품 가격(KRW)
-
충분:
2500 Reactions
EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments. This kit comes with a separate tube of ROX reference dye. EXPRESS One-Step SYBR GreenER SuperMix kits with premixed ROX reference dye are also available.

This kit provides:
• Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase
• The One-Step SuperScript™ III Reverse Transcriptase for one-step qRT-PCR
• SYBR GreenER dye
• UDG carryover protection—uracil-DNA-glycosylase (UDG)/dUTP to reduce carryover contamination

For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
형식Tube
반응 수2500 Reactions
중합효소Taq DNA Polymerase
제품라인SYBR GreenER
제품 유형One-Step qRT-PCR Kit
수량2,500 reactions
샘플 종류RNA
충분2500 Reactions
검출 방법SYBR
PCR 방법1-step RT-qPCR
반응 속도Fast or Standard
Unit SizeEach
구성 및 보관
EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all EXPRESS One-Step SYBR™ GreenER™ Kit, universal, 2,500 reactions FAQs