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SuperScript V VILO Master Mix, Primer-Free, with gDNA Removal
Invitrogen™

SuperScript V VILO Master Mix, Primer-Free, with gDNA Removal

Invitrogen™ SuperScript™ V VILO™ Master Mix, Primer-Free is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis with the flexibility to select your preferred priming strategy.
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Catalog NumberNo. of ReactionsIncludes
1190000150 ReactionsSuperScript V VILO Master Mix (Primer-Free), No RT Control, Water
11900002500 ReactionsSuperScript V VILO Master Mix (Primer-Free), No RT Control, Water
119000032000 ReactionsSuperScript V VILO Master Mix (Primer-Free), No RT Control, Water
1190011150 ReactionsSuperScript V VILO Master Mix (Primer-Free), No RT Control, ezDNase Master Mix, Water
11900112500 ReactionsSuperScript V VILO Master Mix (Primer-Free), No RT Control, ezDNase Master Mix, Water
119001132000 ReactionsSuperScript V VILO Master Mix (Primer-Free), No RT Control, ezDNase Master Mix, Water
119000041250 ReactionsSuperScript V VILO Master Mix (Primer-free) only
Catalog number 11900001
Price (USD)
684.00
Each
No. of Reactions:
50 Reactions
Includes:
SuperScript V VILO Master Mix (Primer-Free), No RT Control, Water
Price (USD)
684.00
Each

Invitrogen™ SuperScript™ V VILO™ Master Mix, Primer-Free is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis with the flexibility to select your preferred priming strategy. Powered by SuperScript™ V Reverse Transcriptase, the optimized master mix combines robust performance across a broad range of RNA samples with simplified reaction setup, enhanced reagent stability, and greater workflow flexibility. SuperScript V VILO Master Mix, Primer-Free is also available with ezDNase™ Master Mix for fast and convenient genomic DNA removal prior to reverse transcription.

Features of SuperScript V VILO Master Mix, Primer-Free include:

  • Broad RNA input range—supports sensitive cDNA synthesis from 0.1 pg to 5 μg of total RNA
  • Robust performance with challenging RNA—efficient cDNA synthesis from highly structured, GC-rich, degraded and inhibitor-containing RNA
  • Fast cDNA synthesis—complete reverse transcription in as little as 1-10 minutes
  • Enhanced reagent stability—supports ambient shipping and room-temperature storage for up to 1 month
  • Simple, flexible workflow—ready-to-use format simplifies reaction setup, while room-temperature assembly and extended stability of assembled reactions enable greater workflow flexibility

SuperScript V VILO Master Mix, Primer-Free combines SuperScript V RT, RNase inhibitor, buffer, and dNTPs in a single formulation while allowing users to select and optimize primers for their specific application. This flexibility supports gene-specific primers, oligo(dT), random primers, or customized primer combinations while maintaining the convenience of a ready-to-use master mix.

SuperScript V VILO Master Mix, Primer-Free with gDNA Removal allows streamlined genomic DNA removal step. The dsDNA-specific ezDNase enzyme removes contaminating genomic DNA in as little as 2 minutes without affecting RNA.

SuperScript V VILO Master Mix, Primer-Free also features a DTT-free, animal origin–free formulation for improved sustainability and simplified reagent handling.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeFirst-Strand cDNA
FormatMaster Mix
IncludesSuperScript V VILO Master Mix (Primer-Free), No RT Control, Water
Inhibitor Tolerance (Simple)High
No. of Reactions50 Reactions
Optimal Reaction Temperature50°C
Product TypeReverse Transcription Reagent
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript V
Ribonuclease H ActivityReduced
Sensitivity0.1 pg total RNA
Shipping ConditionDry Ice, Wet Ice, Ambient
Size (Final Product)Up to 20 kb
Starting MaterialRNA
TechniqueReverse Transcription
ThermostabilityUp to 65°
Concentration5X
Detection MethodqPCR, NGS, PCR
For Use With (Application)Gene Expression, Real Time PCR (qPCR), RT-PCR, cDNA Synthesis, Next-Generation Sequencing (NGS)
FormLiquid
GC-Rich PCR PerformanceHigh
Label or DyeNot Applicable
PCR Method2-step RT-qPCR, RT-PCR
Reaction Speed10 min.
Unit SizeEach
Contents & Storage
• SuperScript V VILO Master Mix, Primer-Free (200 μL)
• SuperScript V VILO No RT Control, Primer-Free (100 μL)
• Nuclease-free water (1.25 mL)

Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 5 months
• Room temperature for up to 1 month

Frequently asked questions (FAQs)

What are the main differences between Invitrogen SuperScript V Reverse Transcriptase and Invitrogen SuperScript V VILO Master Mix?

Invitrogen SuperScript V Reverse Transcriptase is supplied as a stand-alone enzyme with reaction buffer and supports flexible reaction optimization. Invitrogen SuperScript V VILO Master Mix combines all required reaction components into a single formulation to simplify workflow setup and reduce pipetting steps.

Can Invitrogen SuperScript V Reverse Transcriptase products support automated workflows?

Yes. Invitrogen SuperScript V Reverse Transcriptase products include features such as room-temperature reaction setup, benchtop stability of assembled reactions, and simplified workflow options that can support automated and high-throughput workflows.

Can Invitrogen SuperScript V Reverse Transcriptase products support low-input RNA workflows?

Yes. Invitrogen SuperScript V Reverse Transcriptase products are designed to support reverse transcription from low-input RNA samples, including inputs as low as 0.1 pg of total RNA.

Which Invitrogen SuperScript V Reverse Transcriptase product should I choose for RT-qPCR workflows?

For two-step RT-qPCR workflows, Invitrogen SuperScript V VILO Master Mix reagent (Cat. No. 11800001) provides an all-in-one formulation that simplifies reaction setup. Users who prefer custom primer selection may choose Invitrogen SuperScript V VILO Master Mix, Primer-Free (Cat. No. 11900001) or Invitrogen SuperScript V Reverse Transcriptase (Cat. No. 19090050).

Can reaction volumes be scaled when using Invitrogen SuperScript V Reverse Transcriptase products?

Yes. Reaction volumes may be scaled up to 100 µL when using Invitrogen SuperScript V Reverse Transcriptase products provided that reagent ratios are maintained and workflow performance is verified.

View all SuperScript V VILO Master Mix, Primer-Free, with gDNA Removal FAQs