SuperScript V VILO Master Mix, Primer-Free, 50 rxns - FAQs

Invitrogen SuperScript V Reverse Transcriptase is supplied as a stand-alone enzyme with reaction buffer and supports flexible reaction optimization. Invitrogen SuperScript V VILO Master Mix combines all required reaction components into a single formulation to simplify workflow setup and reduce pipetting steps.

Yes. Invitrogen SuperScript V Reverse Transcriptase products include features such as room-temperature reaction setup, benchtop stability of assembled reactions, and simplified workflow options that can support automated and high-throughput workflows.

Yes. Invitrogen SuperScript V Reverse Transcriptase products are designed to support reverse transcription from low-input RNA samples, including inputs as low as 0.1 pg of total RNA.

For two-step RT-qPCR workflows, Invitrogen SuperScript V VILO Master Mix reagent (Cat. No. 11800001) provides an all-in-one formulation that simplifies reaction setup. Users who prefer custom primer selection may choose Invitrogen SuperScript V VILO Master Mix, Primer-Free (Cat. No. 11900001) or Invitrogen SuperScript V Reverse Transcriptase (Cat. No. 19090050).

Yes. Reaction volumes may be scaled up to 100 µL when using Invitrogen SuperScript V Reverse Transcriptase products provided that reagent ratios are maintained and workflow performance is verified.

Invitrogen SuperScript V Reverse Transcriptase products are designed for robust performance with a variety of RNA sample types and may tolerate common reaction inhibitors. Performance may vary depending on sample, inhibitor type, and RNA preparation method.

Invitrogen SuperScript V VILO Master Mix (Cat. No. 11800001) contains a blend of oligo(dT) primers and random hexamer primers. Invitrogen SuperScript V VILO Master Mix, Primer-Free (Cat. No. 11900001) does not contain primers.

Yes. Gene-specific primers can be used with Invitrogen SuperScript V Reverse Transcriptase (Cat. No. 19090050) and Invitrogen SuperScript V VILO Master Mix, Primer-Free (Cat. No. 11900001).

Yes. Invitrogen SuperScript V Reverse Transcriptase and SuperScript V VILO Master Mixes use a DTT-free formulation and do not require users to add DTT during reaction setup.

Yes. An additional no-RT control can be prepared by heating Invitrogen SuperScript V VILO Master Mix at 95°C for 1 minute to inactivate the reverse transcriptase before use.

Yes. Invitrogen SuperScript V Reverse Transcriptase products are designed to support ambient shipping conditions, which may reduce reliance on dry ice or other cold-chain shipping methods.

Invitrogen SuperScript V Reverse Transcriptase enzyme may be stored at room temperature for up to 4 months, while SuperScript V VILO Master Mix products may be stored at room temperature for up to 1 month. For long-term storage through the labeled expiration date, we recommend storing SuperScript V Reverse Transcriptase products at -20°C.

The room-temperature reaction control feature in Invitrogen SuperScript V Reverse Transcriptase products allows greater flexibility during reaction setup, reduces dependence on ice, and can support automated or high-throughput workflows.

Assembled Invitrogen SuperScript V Reverse Transcriptase reactions can remain at room temperature for up to 24 hours when used with probe-based qPCR assays and up to 4 hours when used with SYBR Green-based qPCR assays.

Invitrogen SuperScript V Reverse Transcriptase products include a room-temperature reaction control feature that helps suppress reverse transcription activity during reaction setup. This feature allows assembled reactions to remain stable at room temperature before incubation.

Invitrogen SuperScript V Reverse Transcriptase products combine high sensitivity and broad RNA input compatibility with robust performance and workflow-enhancing features. It supports rapid cDNA synthesis, sensitive detection from very low RNA inputs (down to 100 fg of total RNA), and efficient reverse transcription of difficult or degraded samples, including at elevated temperatures up to 65°C. Workflow features such as room-temperature reaction setup, benchtop stability of assembled reactions, a DTT-free formulation, and enhanced room-temperature reagent stability help simplify handling and reduce dependence on strict cold-chain logistics. Together, these capabilities provide a faster, more flexible reverse transcription workflow while maintaining the sensitivity, consistency, and downstream performance expected from the SuperScript family.

The recommended reaction temperature for Invitrogen SuperScript V Reverse Transcriptase products is 50°C for most applications. SuperScript V Reverse Transcriptase remains active across a temperature range of 37-65°C, allowing optimization for different RNA templates and primer strategies. Lower temperatures may improve primer annealing, while higher temperatures may benefit GC-rich or structured RNA and improve gene-specific priming.

Yes. Invitrogen SuperScript V Reverse Transcriptase is engineered for high thermostability and can support reverse transcription of GC-rich or highly structured RNA templates. For challenging RNA templates, reaction temperatures up to 65°C may improve cDNA yield and transcript representation.

Yes. Invitrogen SuperScript V Reverse Transcriptase products are designed to support cDNA synthesis from a range of RNA qualities, including degraded RNA samples. For degraded RNA samples, we recommend using short qPCR amplicons, avoiding targets near the 5′ end of long transcripts, including appropriate reference genes, and following the reverse transcription recommendations provided in the product documentation.

Invitrogen SuperScript V Reverse Transcriptase and Invitrogen SuperScript V VILO Master Mix products support RNA inputs from 0.1 pg to 5 µg of total RNA or up to 500 ng of mRNA.

Invitrogen SuperScript V Reverse Transcriptase products support reverse transcription in as little as 1-10 min depending on the workflow and target abundance. For most applications, we recommend a 10 min reverse transcription incubation step.

Yes. Invitrogen SuperScript V Reverse Transcriptase products are manufactured as Animal Origin Free (AOF) formulations and do not contain components of animal origin.

Invitrogen SuperScript V Reverse Transcriptase (Cat. No. 19090050) is supplied with a 5X reaction buffer and is suitable for users who want maximum flexibility for reaction setup and primer selection. Invitrogen SuperScript V VILO Master Mix (Cat. No. 11800001) combines reverse transcriptase enzyme, RNase inhibitor, buffer, MgCl₂, dNTPs, and an optimized primer blend in a one-tube formulation, optimized for broad transcript coverage in two step RT-qPCR applications. SuperScript V VILO Master Mix, Primer-Free (Cat. No. 11900001), allows users to add their own primers. Versions with integrated genomic DNA removal are also available (Cat. Nos. 11800111 and 11900111).

Invitrogen SuperScript V Reverse Transcriptase products support first-strand cDNA synthesis workflows, including RT-qPCR, RT-PCR, gene expression analysis, RNA sequencing library preparation, and analysis of low-input or challenging RNA samples.