'Human osteoblasts produce interleukin-6 (IL-6) and respond to IL-6 in the presence of soluble IL-6 receptor (sIL-6R), but the cell surface expression of IL-6R and the mechanism of sIL-6R production are largely unknown. Three different human osteoblast-like cell lines (MG-63, HOS, and SaOS-2) and bone marrow-derived primary human osteoblasts expressed ... More
The transcriptional regulating protein of 132 kDa (TReP-132) enhances P450scc gene transcription through interaction with steroidogenic factor-1 in human adrenal cells.
'The human P450scc gene is regulated by the tissue-specific orphan nuclear receptor, steroidogenic factor-1 (SF-1), which plays a key role in several physiologic processes including steroid synthesis, adrenal and gonadal development, and sexual differentiation. Several studies have demonstrated the interaction of SF-1 with different proteins. However, it is clear that ... More
Expression profiling and gene discovery in the mouse lens.
AuthorsWride MA, Mansergh FC, Adams S, Everitt R, Minnema SE, Rancourt DE, Evans MJ,
JournalMol Vis
PubMed ID12942050
'PURPOSE: Defects in the development and physiology of the lens can result in cataracts (opacification of the lens), which are currently treatable only by surgical removal. The lens is also an excellent system for understanding fundamental biological processes such as cellular differentiation and ageing. Here, microarrays have been used to ... More
Glucocorticoid Activation of Na+/H+ Exchanger Isoform 3 Revisited. THE ROLES OF SGK1 AND NHERF2.
Authors Yun C Chris; Chen Yueping; Lang Florian;
JournalJ Biol Chem
PubMed ID11751930
'The stimulative effect of glucocorticoids on intestinal salt and water absorption has been known for more than two decades. However, molecular mechanisms underlying this activation remain elusive. Previous studies showed that methylprednisolone specifically increased Na(+)/H(+) exchanger isoform (NHE) 3 mRNA in ileum and kidney without affecting NHE1 mRNA levels. These ... More
The gene expression fingerprint of human heart failure.
Authors Tan Fen-Lai; Moravec Christine S; Li Jianbo; Apperson-Hansen Carolyn; McCarthy Patrick M; Young James B; Bond Meredith;
JournalProc Natl Acad Sci U S A
PubMed ID12177426
'Multiple pathways are responsible for transducing mechanical and hormonal stimuli into changes in gene expression during heart failure. In this study our goals were (i) to develop a sound statistical method to establish a comprehensive cutoff point for identification of differentially expressed genes, (ii) to identify a gene expression fingerprint ... More
Previously uncharacterized isoforms of divalent metal transporter (DMT)-1: Implications for regulation and cellular function.
Authors Hubert Nadia; Hentze Matthias W;
JournalProc Natl Acad Sci U S A
PubMed ID12209011
'Divalent metal transporter 1 (DMT1) mediates apical iron uptake into duodenal enterocytes and also transfers iron from the endosome into the cytosol after cellular uptake via the transferrin receptor. Hence, mutations in DMT1 cause systemic iron deficiency and anemia. DMT1 mRNA levels are increased in the duodenum of iron-deficient animals. ... More
From the Cover: Mutations in Mcoln3 associated with deafness and pigmentation defects in varitint-waddler (Va) mice.
Authors Di Palma Federica; Belyantseva Inna A.; Kim Hung J.; Vogt Thomas F.; Kachar Bechara; Noben-Trauth Konrad;
JournalProc Natl Acad Sci U S A
PubMed ID12403827
'Deafness in spontaneously occurring mouse mutants is often associated with defects in cochlea sensory hair cells, opening an avenue to systematically identify genes critical for hair cell structure and function. The classical semidominant mouse mutant varitint-waddler (Va) exhibits early-onset hearing loss, vestibular defects, pigmentation abnormalities, and perinatal lethality. A second ... More
Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization.
'Amelogenin is an extracellular matrix protein secreted by ameloblasts and is a major component of enamel matrix. Recently, in addition to their role in enamel formation, the biological activity of enamel proteins in the process of cell differentiation has recently become widely appreciated. In this study, we examined the biological ... More
Inducible expression of a dominant negative DNA polymerase-gamma depletes mitochondrial DNA and produces a rho0 phenotype.
AuthorsJazayeri M, Andreyev A, Will Y, Ward M, Anderson CM, Clevenger W,
JournalJ Biol Chem
PubMed ID12645575
'We report the inducible, stable expression of a dominant negative form of mitochondria-specific DNA polymerase-gamma to eliminate mitochondrial DNA (mtDNA) from human cells in culture. HEK293 cells were transfected with a plasmid encoding inactive DNA polymerase-gamma harboring a D1135A substitution (POLGdn). The cells rapidly lost mtDNA (t1/2 = 2-3 days) ... More
Critical contribution of linker proteins to raf kinase activation.
Authors Anselmo Anthony N; Bumeister Ron; Thomas Jackie M; White Michael A;
JournalJ Biol Chem
PubMed ID11741918
'Genetic analysis of Ras signaling has unveiled the participation of non-enzymatic accessory proteins in signal transmission. These proteins, KSR, CNK, and Sur-8, can interact with multiple core components of the Ras/MAP kinase cascade and may contribute to the structural organization of this cascade. However, the precise biochemical nature of the ... More
GlcNAc 2-epimerase can serve a catabolic role in sialic acid metabolism.
'Sialic acid is a major determinant of carbohydrate-receptor interactions in many systems pertinent to human health and disease. N-Acetylmannosamine (ManNAc) is the first committed intermediate in the sialic acid biosynthetic pathway; thus, the mechanisms that control intracellular ManNAc levels are important regulators of sialic acid production. UDP-GlcNAc 2-epimerase and GlcNAc ... More
Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes.
Authors Vazquez G; Lievremont J P; St J Bird G; Putney J W Jr;
JournalProc Natl Acad Sci U S A
PubMed ID11553786
'Mammalian Trp proteins are candidates for plasma membrane calcium channels regulated by receptor activation or by intracellular calcium store depletion [capacitative calcium entry (CCE)]. One extensively investigated member of the Trp family, the human Trp3 (hTrp3), behaves as a receptor-activated, calcium-permeable, nonselective cation channel when expressed in cell lines and ... More
Helical apolipoproteins stabilize ATP-binding cassette transporter A1 by protecting it from thiol protease-mediated degradation.
Authors Arakawa Reijiro; Yokoyama Shinji;
JournalJ Biol Chem
PubMed ID11950847
'ATP-binding cassette transporter (ABC) A1 was increased by apolipoprotein A-I without an increase of its message in THP-1 cells. The pulse label study demonstrated that apoA-I retarded degradation of ABCA1. Similar changes were demonstrated by apoA-II, but the effect of high density lipoprotein was almost negligible on the basis of ... More
Transcriptional regulation of the transforming growth factor beta type II receptor gene by histone acetyltransferase and deacetylase is mediated by NF-Y in human breast cancer cells.
Authors Park Seok Hee; Lee Sae Ra; Kim Byung Chul; Cho Eun Ah; Patel Sejal P; Kang Hee-Bum; Sausville Edward A; Nakanishi Osamu; Trepel Jane B; Lee Byoung Ick; Kim Seong-Jin;
JournalJ Biol Chem
PubMed ID11744689
'Transcriptional repression of the transforming growth factor-beta (TGF-beta) type II receptor (TbetaRII) gene is one of several mechanisms leading to TGF-beta resistance. Previously, we have shown that MS-275, a synthetic inhibitor of histone deacetylase (HDAC), specifically induces the expression of the TbetaRII gene and restores the TGF-beta signaling in human ... More
A Calcium-Responsive Transcription Factor, CaRF, that Regulates Neuronal Activity-Dependent Expression of BDNF.
Authors Tao Xu; West Anne E; Chen Wen G; Corfas Gabriel; Greenberg Michael E;
JournalNeuron
PubMed ID11832226
'Transcription of the brain-derived neurotrophic factor (BDNF) gene is regulated in a calcium- and neuron-selective manner; however, the mechanisms that underlie this selectivity are not known. We have characterized a new calcium-response element, CaRE1, that is required for activity-dependent transcription of BDNF exon III and have cloned a transcription factor, ... More
RNA cargoes associating with FMRP reveal deficits in cellular functioning in Fmr1 null mice.
AuthorsMiyashiro KY, Beckel-Mitchener A, Purk TP, Becker KG, Barret T, Liu L, Carbonetto S, Weiler IJ, Greenough WT, Eberwine J,
JournalNeuron
PubMed ID12575950
'The Fragile X mental retardation-1 (Fmr1) gene encodes a multifunctional protein, FMRP, with intrinsic RNA binding activity. We have developed an approach, antibody-positioned RNA amplification (APRA), to identify the RNA cargoes associated with the in vivo configured FMRP messenger ribonucleoprotein (mRNP) complex. Using APRA as a primary screen, putative FMRP ... More
Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation.
AuthorsSuzuki T, Okada Y, Semba S, Orba Y, Yamanouchi S, Endo S, Tanaka S, Fujita T, Kuroda S, Nagashima K, Sawa H,
JournalJ Biol Chem
PubMed ID15843383
'The human polyomavirus JC virus (JCV) is the causative agent of a fatal demyelinating disease, progressive multifocal leukoencephalopathy, and encodes six major proteins, including agnoprotein. Agnoprotein colocalizes with microtubules in JCV-infected cells, but its function is not fully understood. We have now identified fasciculation and elongation protein zeta 1 (FEZ1) ... More
Congenital disorders of glycosylation type Ig is defined by a deficiency in dolichyl-P-mannose:Man7GlcNAc2-PP-dolichyl mannosyltransferase.
'Type I congenital disorders of glycosylation (CDG I) are diseases presenting multisystemic lesions including central and peripheral nervous system deficits. The disease is characterized by under-glycosylated serum glycoproteins and is caused by mutations in genes encoding proteins involved in the stepwise assembly of dolichol-oligosaccharide used for protein N-glycosylation. We report ... More
Complex DNA melting profiles of small PCR products revealed using SYBR Green I.
AuthorsLi W, Xi B, Yang W, Hawkins M, Schubart UK,
JournalBiotechniques
PubMed ID14579734
None
Visualization and identification of IL-7 producing cells in reporter mice.
AuthorsMazzucchelli RI, Warming S, Lawrence SM, Ishii M, Abshari M, Washington AV, Feigenbaum L, Warner AC, Sims DJ, Li WQ, Hixon JA, Gray DH, Rich BE, Morrow M, Anver MR, Cherry J, Naf D, Sternberg LR, McVicar DW, Farr AG, Germain RN, Rogers K, Jenkins NA, Copeland NG, Durum SK,
JournalPLoS One
PubMed ID19907640
Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein) accumulated inside ... More
SIRT3, a human SIR2 homologue, is an NAD-dependent deacetylase localized to mitochondria.
The SIR2 (silent information regulator 2) gene family has diverse functions in yeast including gene silencing, DNA repair, cell-cycle progression, and chromosome fidelity in meiosis and aging. Human homologues, termed sirtuins, are highly conserved but are of unknown function. We previously identified a large imprinted gene domain on 11p15.5 and ... More
Causal Relationship between the Loss of RUNX3 Expression and Gastric Cancer.
Authors Li Qing Lin; Ito Kosei; Sakakura Chohei; Fukamachi Hiroshi; Inoue Ken ichi; Chi Xin Zi; Lee Kwang Youl; Nomura Shintaro; Lee Chang Woo; Han Sang Bae; Kim Hwan Mook; Kim Wun Jae; Yamamoto Hiromitsu; Yamashita Namiko; Yano Takashi; Ikeda Toshio; Itohara Shigeyoshi; Inazawa Johji; Abe Tatsuo; Hagiwara Akeo; Yamagishi Hisakazu; Ooe Asako; Kaneda Atsushi; Sugimura Takashi; Ushijima Toshikazu; Bae Suk Chul; Ito Yoshiaki;
JournalCell
PubMed ID11955451
Runx3/Pebp2alphaC null mouse gastric mucosa exhibits hyperplasias due to stimulated proliferation and suppressed apoptosis in epithelial cells, and the cells are resistant to growth-inhibitory and apoptosis-inducing action of TGF-beta, indicating that Runx3 is a major growth regulator of gastric epithelial cells. Between 45% and 60% of human gastric cancer cells ... More
Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin.
Authors Mukouyama Yoh suke; Shin Donghun; Britsch Stefan; Taniguchi Masahiko; Anderson David J;
JournalCell
PubMed ID12086669
Nerves and blood vessels are branched structures, but whether their branching patterns are established independently or coordinately is not clear. Here we show that arteries, but not veins, are specifically aligned with peripheral nerves in embryonic mouse limb skin. Mutations that eliminate peripheral sensory nerves or Schwann cells prevent proper ... More
Cloning and Characterization of a Cryptic Haloacid Dehalogenase from Burkholderia cepacia MBA4
AuthorsJ. Tsang and L. Sam
JournalJ Bacteriol
PubMed ID10498712
Burkholderia cepacia MBA4 has been shown to produce a single dehalogenase batch culture.Moreover, other cryptic dehalogenases were also detected when the cells were grown in continuousculture. In this paper, we report the cloning and characterization of one of the cryptic dehalogenases inMBA4. This cryptic haloacid dehalogenase, designated Chd1, was expressed ... More
Canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmission.
AuthorsWang W, Hall AE, O'Malley R, Bleecker AB,
JournalProc Natl Acad Sci U S A
PubMed ID12509505
Ethylene signaling in plants is mediated by a family of receptors related to bacterial two-component histidine kinases. Of the five members of the Arabidopsis ethylene receptor family, members of subfamily I (ETR1 and ERS1) contain completely conserved histidine kinase domains, whereas members of subfamily II (ETR2, EIN4, and ERS2) lack ... More
Contribution of Fas to diabetes development.
AuthorsSavinov AY, Tcherepanov A, Green EA, Flavell RA, Chervonsky AV,
JournalProc Natl Acad Sci U S A
PubMed ID12525697
Fas (Tnfrsf6, Apo-1, CD95) is a death receptor involved in apoptosis induced in many cell types. Fas have been shown to be expressed by insulin-producing beta cells in mice and humans. However, the importance of Fas in the development of autoimmune diabetes remains controversial. To further evaluate the importance of ... More
Roles of neurotransmitter in synapse formation: development of neuromuscular junctions lacking choline acetyltransferase.
Activity-dependent and -independent signals collaborate to regulate synaptogenesis, but their relative contributions are unclear. Here, we describe the formation of neuromuscular synapses at which neurotransmission is completely and specifically blocked by mutation of the neurotransmitter-synthesizing enzyme choline acetyltransferase. Nerve terminals differentiate extensively in the absence of neurotransmitter, but neurotransmission plays ... More
A role for the granzyme B inhibitor serine protease inhibitor 6 in CD8+ memory cell homeostasis.
AuthorsPhillips T, Opferman JT, Shah R, Liu N, Froelich CJ, Ashton-Rickardt PG,
JournalJ Immunol
PubMed ID15356127
Generation and maintenance of protective immunological memory is the goal of vaccination programs. It has recently become clear that CD8+ memory T cells are derived directly from CTLs. The mechanisms underlying this transformation and the subsequent survival of memory cells are not completely understood. However, some effector molecules required by ... More
P21 functions to maintain quiescence of p27-deficient hepatocytes.
AuthorsKwon YH, Jovanovic A, Serfas MS, Kiyokawa H, Tyner AL,
JournalJ Biol Chem
PubMed ID12202477
Hepatocytes rarely proliferate in the healthy adult liver. We explored the roles of the cyclin kinase inhibitors p21 and p27 in maintaining hepatocyte quiescence. p27 is expressed throughout the wild-type liver, but the related protein p21 was not detected. However, p21 was detected in livers of p27-deficient mice. Increased p21 ... More
Suppression of postischemic hippocampal nerve growth factor expression by a c-fos antisense oligodeoxynucleotide.
AuthorsCui JK, Hsu CY, Liu PK,
JournalJ Neurosci
PubMed ID9952411
We examined the uptake and distribution of an antisense phosphorothioated oligodeoxynucleotide (s-ODN) to c-fos, rncfosr115, infused into the left cerebral ventricle of male Long-Evans rats and the effect of this s-ODN on subsequent Fos, NGF, neurotrophin-3 (NT-3), and actin expression. To establish the uptake and turnover of s-ODN in the ... More
Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses.
The present study shows that the H19 and Igf2r genes, which are imprinted and expressed solely from maternal alleles, are expressed in an unregulatable manner in mouse uniparental, androgenetic, and parthenogenetic fetuses at day 9.5 of gestation. In the androgenetic fetuses, the H19 and Igf2r genes were respectively expressed at ... More
Brn-4 Transcription Factor Expression Targeted to the Early Developing Mouse Pancreas Induces Ectopic Glucagon Gene Expression in Insulin-producing beta Cells.
Authors Hussain Mehboob A; Miller Christopher P; Habener Joel F;
JournalJ Biol Chem
PubMed ID11834725
The endocrine pancreas is comprised of beta and alpha cells producing the glucostatic hormones insulin and glucagon, respectively, and arises during development by the differentiation of stem/progenitor cells in the foregut programmed by the beta cell lineage-specific homeodomain protein Idx-1. Brain-4 (Brn-4) is expressed in the pancreatic anlaga of the ... More
Transcriptional Regulation of Mouse delta -Opioid Receptor Gene. ROLE OF IKAROS IN THE STIMULATED TRANSCRIPTION OF MOUSE delta -OPIOID RECEPTOR GENE IN ACTIVATED T CELLS.
Authors Sun Ping; Loh Horace H;
JournalJ Biol Chem
PubMed ID11823468
delta-Opioid receptors (DOR) present on T cells have been shown to mediate the immunomodulatory effects of endogenous and synthetic DOR agonists on T cells. Considerable evidence indicates that there is stimulated transcription of DOR gene in activated T cells, which is correlated with augmented expression of DOR and enhanced capacity ... More
Role of the thyroid-stimulating hormone receptor signaling in development and differentiation of the thyroid gland.
Authors Postiglione M P; Parlato R; Rodriguez-Mallon A; Rosica A; Mithbaokar P; Maresca M; Marians R C; Davies T F; Zannini M S; De Felice M; Di Lauro R;
JournalProc Natl Acad Sci U S A
PubMed ID12432093
The thyroid-stimulating hormonethyrotropin (TSH) is the most relevant hormone in the control of thyroid gland physiology in adulthood. TSH effects on the thyroid gland are mediated by the interaction with a specific TSH receptor (TSHR). We studied the role of TSHTSHR signaling on gland morphogenesis and differentiation in the mouse ... More
Involvement of receptor-interacting protein 2 in innate and adaptive immune responses.
Authors Chin Arnold I; Dempsey Paul W; Bruhn Kevin; Miller Jeff F; Xu Yang; Cheng Genhong;
JournalNature
PubMed ID11894097
Host defences to microorganisms rely on a coordinated interplay between the innate and adaptive responses of immunity. Infection with intracellular bacteria triggers an immediate innate response requiring macrophages, neutrophils and natural killer cells, whereas subsequent activation of an adaptive response through development of T-helper subtype 1 cells (TH1) proceeds during ... More
Ceramide mediates age-associated increase in macrophage cyclooxygenase-2 expression.
Authors Claycombe Kate J; Wu Dayong; Nikolova-Karakashian Mariana; Palmer Helen; Beharka Alison; Paulson K Eric; Meydani Simin Nikbin;
JournalJ Biol Chem
PubMed ID12072440
Previously, we showed that macrophages (MØ) from old mice have significantly higher levels of lipopolysaccharide (LPS)-induced prostaglandin E(2) (PGE(2)) production than young mice, due to increased cyclooxygenase-2 (COX-2) mRNA levels. The aim of the current study was to determine the underlying mechanisms of age-associated increase in COX-2 gene expression. The ... More
Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication.
Authors Goncalves Joao; Silva Frederico; Freitas-Vieira Acilino; Santa-Marta Mariana; Malhó Rui; Yang Xiaoyu; Gabuzda Dana; Barbas Carlos 3rd;
JournalJ Biol Chem
PubMed ID12039955
Human immunodeficiency virus type 1 (HIV-1)-encoded Vif protein is important for viral replication and infectivity. Vif is a cytoplasmic protein that acts during virus assembly by an unknown mechanism, enhancing viral infectivity. The action of Vif in producer cells is essential for the completion of proviral DNA synthesis following virus ... More
Endothelial argininosuccinate synthase mRNA 5'-untranslated region diversity. Infrastructure for tissue-specific expression.
Authors Pendleton Laura C; Goodwin Bonnie L; Flam Brenda R; Solomonson Larry P; Eichler Duane C;
JournalJ Biol Chem
PubMed ID11967259
Based on the integral role that argininosuccinate synthase (AS) plays in the production of nitric oxide in vascular endothelial cells and urea in liver, an analysis was carried out to determine whether signals reside in the AS mRNA to account for tissue differences in AS function and location. Reverse transcriptase-PCR ... More
Extensive repertoire of membrane-bound and soluble dendritic cell-specific ICAM-3-grabbing nonintegrin 1 (DC-SIGN1) and DC-SIGN2 isoforms. Inter-individual variation in expression of DC-SIGN transcripts.
Expression in dendritic cells (DCs) of DC-SIGN, a type II membrane protein with a C-type lectin ectodomain, is thought to play an important role in establishing the initial contact between DCs and resting T cells. DC-SIGN is also a unique type of human immunodeficiency virus-1 (HIV-1) attachment factor and promotes ... More
Regulation of hyaluronidase activity by alternative mRNA splicing.
Authors Lokeshwar Vinata B; Schroeder Grethchen L; Carey Robert I; Soloway Mark S; Iida Naoko;
JournalJ Biol Chem
PubMed ID12084718
Hyaluronidase is a hyaluronic acid-degrading endoglycosidase that is present in many toxins and the levels of which are elevated in cancer. Increased concentration of HYAL1-type hyaluronidase correlates with tumor progression and is a marker for grade (G) 2 or 3 bladder cancer. Using bladder tissues and cells, prostate cancer cells, ... More
Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that transfers N-acetylgalactosamine to glucuronic acid in initiation and elongation of chondroitin sulfate synthesis.
We found a novel glycosyltransferase gene having a hypothetical beta 1,4-galactosyltransferase motif (GenBank accession number ) by a BLAST search and cloned its full-length open reading frame using the 5'-rapid amplification of cDNA ends method. The truncated form was expressed in insect cells as a soluble enzyme. It transferred N-acetylgalactosamine, ... More
Phosphorylation and Dimerization Regulate Nucleocytoplasmic Shuttling of Mammalian STE20-like Kinase (MST).
Authors Lee Kyung-Kwon; Yonehara Shin;
JournalJ Biol Chem
PubMed ID11805089
Mammalian STE20-like kinase (MST) is a member of the yeast STE20-related kinase family and proteolytically activated by caspase during apoptosis. However, its other cellular functions are not known, including its activation mechanism, substrate(s), and subcellular localization. In this report, using anti-MST monoclonal antibodies, we clearly show that endogenous MST is ... More
Wnt-3A enhances bone morphogenetic protein-2-mediated chondrogenesis of murine C3H10T1/2 mesenchymal cells.
We have recently reported the chondrogenic effect of bone morphogenetic protein-2 (BMP-2) in high density cultures of the mouse multipotent mesenchymal C3H10T1/2 cell line and have shown the functional requirement of the cell-cell adhesion molecule N-cadherin in BMP-2-induced chondrogenesis in vitro (Denker, A. E., Nicoll, S. B., and Tuan, R. ... More