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Invitrogen
CD44 Monoclonal Antibody (IM7), PE, eBioscience™
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FIGURE: 1 / 99
CD44 Antibody (12-0441-81) in Flow
Differential expression of Anti-Human/Mouse CD44 PE on mouse bone marrow cells. BALB/c bone marrow cells were stained with 0.06 µg of Anti-Human/Mouse CD44 PE. Total viable cells were gated further on their light scatter profile. The lymphoid lineage cells (R2) express Anti-Human/Mouse CD44 PE at an intermediate level, while the myeloid lineage cells (R3 & R4) display much higher levels of Anti-Human/Mouse CD44 PE surface expression. The overlay histogram on each panel (green) is the autofluorescence of cells.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-0441-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Bovine,
Fruit fly,
Human,
Mammal,
Mouse,
Non-human primate,
Rabbit,
Rat
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
IM7
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.
Applications Reported: The IM7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cd44; CD44 molecule; CD44 molecule (Indian blood group); CD44 precursor; CD44R1; CDw44 antigen precursor; cell surface glycoprotein CD44; cell-cell and cell-matrix adhesion interaction glycoprotein isoform; cell-surface glycoprotein; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; epican; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; Hermes antigen; Hermes antigen gp90 homing receptor precursor; homing cell adhesion molecule; homing function and Indian blood group system; homing receptor; HUTCH-I; hyaluronan receptor; hyaluronan-binding protein; hyaluronate receptor; In(Lu) related-p80; Indian blood group antigen; lymphocyte antigen 24; PGP I; PGP-I; PGPI; phagocyte glycoprotein 1; phagocytic glycoprotein 1; phagocytic glycoprotein I; phosphoglycoprotein Pgp-1 (Ly24.2) precursor; sCD 44; sCD44; soluble CD 44; soluble CD44; unnamed protein product
Gene Aliases: CDW44; CSPG8; ECM-III; ECMR-III; H-CAM; HCELL; HERMES; Hermes-1; HUTCH-1; HUTCH-I; LHR; Ly-24; MC56; MDU2; MDU3; MIC4; Pgp-1; Pgp1
UniProt ID: (Human) P16070, (Mouse) P15379
Entrez Gene ID: (Human) 960, (Mouse) 12505
transmembrane signaling receptor activity
cytokine receptor activity
protein binding
collagen binding
hyaluronic acid binding
signaling receptor activity
cargo receptor activity
type II transforming growth factor beta receptor binding
epidermal growth factor receptor binding
channel regulator activity
protein kinase binding
cytokine binding
phosphoprotein binding
regulation of cell growth
branching involved in ureteric bud morphogenesis
wound healing involved in inflammatory response
positive regulation of adaptive immune response
negative regulation of mature B cell apoptotic process
receptor-mediated endocytosis
inflammatory response
cell adhesion
anatomical structure morphogenesis
positive regulation of gene expression
Wnt signaling pathway
cell migration
cytokine-mediated signaling pathway
hyaluronan catabolic process
neuron projection development
positive regulation of neutrophil apoptotic process
positive regulation of heterotypic cell-cell adhesion
macrophage fusion
T cell activation
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
wound healing, spreading of cells
cellular response to fibroblast growth factor stimulus
negative regulation of regulatory T cell differentiation
system development
negative regulation of inflammatory response
NK T cell activation
branching involved in prostate gland morphogenesis
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
regulation of modification of postsynaptic structure
postsynapse organization
positive regulation of monocyte aggregation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of leukocyte apoptotic process
regulation of lamellipodium morphogenesis
negative regulation of CD4-positive, alpha-beta T cell proliferation
endocytosis
cell-matrix adhesion
regulation of apoptotic process
cartilage development
cell-cell adhesion
carbohydrate derivative transport
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