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Invitrogen
CD44H Monoclonal Antibody (OX49), PE, eBioscience™
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Product Details
12-0444-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX49
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This OX49 monoclonal antibody recognizes rat CD44H, which is also known as homing-associated cell adhesion molecule (H-CAM) or phagocytic glycoprotein-1 (Pgp-1). This isoform of CD44 is the cell surface receptor for hyaluronate, a component of the extracellular matrix. CD44H is highly expressed on hematopoietic and non-hematopoietic cells, and exists as multiple splice variants. CD44H plays a critical role in lymphocyte activation and homing.
Applications Reported: This OX49 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX49 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD44; CD44 antigen; Cell surface glycoprotein CD44 (hyaluronate binding protein); ECMR-III; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; HUTCH-I; Hyaluronate receptor; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I
Gene Aliases: Cd44; CD44A; METAA; RHAMM
UniProt ID: (Rat) P26051
Entrez Gene ID: (Rat) 25406
regulation of cell growth
branching involved in ureteric bud morphogenesis
blood vessel maturation
wound healing involved in inflammatory response
negative regulation of mature B cell apoptotic process
inflammatory response
cell adhesion
positive regulation of gene expression
response to organic cyclic compound
Wnt signaling pathway
cell migration
hyaluronan catabolic process
neuron projection development
positive regulation of neutrophil apoptotic process
positive regulation of peptidyl-serine phosphorylation
response to vitamin A
positive regulation of heterotypic cell-cell adhesion
macrophage fusion
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
cellular response to fibroblast growth factor stimulus
positive regulation of peptidyl-tyrosine phosphorylation
cartilage development
branching involved in prostate gland morphogenesis
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
positive regulation of monocyte aggregation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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