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Invitrogen
CD44H Monoclonal Antibody (OX49), PE, eBioscience™
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-0444-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX49
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This OX49 monoclonal antibody recognizes rat CD44H, which is also known as homing-associated cell adhesion molecule (H-CAM) or phagocytic glycoprotein-1 (Pgp-1). This isoform of CD44 is the cell surface receptor for hyaluronate, a component of the extracellular matrix. CD44H is highly expressed on hematopoietic and non-hematopoietic cells, and exists as multiple splice variants. CD44H plays a critical role in lymphocyte activation and homing.
Applications Reported: This OX49 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX49 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: alternatively spliced; CD44 molecule (Indian blood group); Cell surface glycoprotein CD44 (hyaluronate binding protein); cell surface receptor; ECMR-III; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hermes antigen; HUTCH-I; hyaluronate receptor; PGP-1; PGP-I; phagocytic glycoprotein 1; phagocytic glycoprotein I
Gene Aliases: CD44A; METAA; RHAMM
UniProt ID: (Rat) P26051
Entrez Gene ID: (Rat) 25406
transmembrane signaling receptor activity
cytokine receptor activity
type II transforming growth factor beta receptor binding
epidermal growth factor receptor binding
protein binding
hyaluronic acid binding
protein kinase binding
cytokine binding
signaling receptor activity
cargo receptor activity
phosphoprotein binding
regulation of cell growth
branching involved in ureteric bud morphogenesis
blood vessel maturation
wound healing involved in inflammatory response
negative regulation of mature B cell apoptotic process
endocytosis
receptor-mediated endocytosis
inflammatory response
cell adhesion
anatomical structure morphogenesis
positive regulation of gene expression
Wnt signaling pathway
cell migration
cytokine-mediated signaling pathway
hyaluronan catabolic process
neuron projection development
response to nutrient levels
positive regulation of neutrophil apoptotic process
response to vitamin A
positive regulation of heterotypic cell-cell adhesion
macrophage fusion
T cell activation
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
wound healing, spreading of cells
cellular response to fibroblast growth factor stimulus
negative regulation of regulatory T cell differentiation
system development
negative regulation of inflammatory response
NK T cell activation
cartilage development
branching involved in prostate gland morphogenesis
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
regulation of modification of postsynaptic structure
postsynapse organization
positive regulation of monocyte aggregation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of leukocyte apoptotic process
regulation of lamellipodium morphogenesis
negative regulation of CD4-positive, alpha-beta T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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