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Invitrogen
CD355 (CRTAM) Monoclonal Antibody (Cr24.1), PE, eBioscience™
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FIGURE: 1 / 1
CD355 (CRTAM) Antibody (12-3559-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated for 4 hours with the Cell Stimulation Cocktail (Product # 00-4970-93) (right). Cells were then stained with CD8 Monoclonal Antibody, APC (Product # 17-0088-42) and CD355 Monoclonal Antibody, PE. Cells in the lymphocyte gate were used for analysis.
Product Details
12-3559-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
Cr24.1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This monoclonal antibody Cr24.1 reacts with human CD355, also known as Class-I MHC-restricted T-cell-associated molecule (CRTAM). CD355 usually exists as a homodimer, however, it forms a heterodimer with its ligand Necl-2. CD355 is transiently expressed by activated NK, NKT, gamma delta T, and CD8+ T cells. CD355 is also expressed by Purkinje neurons. Human CD355 is involved in immunosurveillance of cancer cells, gamma delta T cell survival, cell polarity, retention of lymphocytes in lymph nodes, promotion of cytotoxicity in NK cells, and IFN gamma secretion from CD8+ T cells. Cr24.1 has been reported to have antagonist activity.
Applications Reported: This CR24.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This CR24.1 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
Target Information
Class-I MHC-restricted T cell-associated molecule (CRTAM) is a gene located on chromosome 11q24.2, encoding a member of the immunoglobulin superfamily that plays a crucial role in immune cell interactions. CRTAM is expressed on activated CD8+ T cells, natural killer (NK) cells, and some subsets of CD4+ T cells, where it is involved in promoting cell adhesion, cytokine production, and cytotoxic responses. The protein binds to Necl-2 (CD112), mediating intercellular communication that influences immune responses, particularly in the context of viral infections and tumor surveillance. CRTAM's involvement in directing immune cell activity highlights its importance in the regulation of adaptive and innate immunity. Research into CRTAM is exploring its potential as a therapeutic target for modulating immune functions, aiming to enhance immune responses against cancer and infectious diseases or to attenuate autoimmune reactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD355; CD355 antigen; Class-I MHC-restricted T-cell-associated molecule; Cytotoxic and regulatory T-cell molecule; immunoglobulin supergene family member; unnamed protein product
Gene Aliases: CD355; CRTAM
UniProt ID: (Human) O95727
Entrez Gene ID: (Human) 56253
establishment of T cell polarity
positive regulation of cytokine production
T cell mediated cytotoxicity
adaptive immune response
detection of tumor cell
immune system process
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
cell recognition
positive regulation of interferon-gamma production
regulation of T cell differentiation
positive regulation of natural killer cell mediated cytotoxicity
negative regulation of activated T cell proliferation
activated T cell proliferation
regulation of T cell activation
detection of stimulus
lymphocyte migration into lymphoid organs
regulation of CD8-positive, alpha-beta T cell activation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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