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          • Primary Antibodies ›
          • ZAP70/Syk Antibodies

          Invitrogen

          Phospho-ZAP70/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), PE, eBioscience™

          1 Published Figure
          4 References
          View all (11) ZAP70/Syk antibodies

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          Cite Phospho-ZAP70/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), PE, eBioscience™

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (1)
          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody (12-9006-41) in Flow

          Intracellular staining of untreated (orange histogram) or 5-minute hydrogen peroxide-activated sodium pervanadate-treated (purple histogram) mouse spleen cells with Anti-Human/Mouse phospho-ZAP-70/SYK (Y319/Y352) PE using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Lymphocytes in the CD3+ (left) or B220+ (right) gates were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)
          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)

          Product Details

          12-9006-41

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL (0.03 µg)/test
          View 4 publications 4 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG2b, kappa

          Recommended Isotype Control

          Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          n3kobu5

          Conjugate

          PE PE PE
          • APC 
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Request custom conjugation

          Excitation/Emission Max

          565/576 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2572664

          Product Specific Information

          Description: This n3kobu5 monoclonal antibody recognizes human and mouse zeta chain-associated protein of 70 kD (also known as ZAP-70) and spleen tyrosine kinase (also known as SYK) when phosphorylated on Y319 and Y352, respectively. ZAP-70 and SYK are members of the SYK protein tyrosine kinase (PTK) family that are phosphorylated and activated by Src family PTKs. ZAP-70/SYK Y319/Y352 are located in the so-called interdomain of ZAP-70/SYK (between the N-terminal dual SH2 domains and the C-terminal kinase domain).

          Phosphorylation of ZAP-70 Y319 by Lck is necessary for T cell receptor (TCR)-dependent association of ZAP-70 with Lck and phospholipase C gamma and subsequent activation of calcium-dependent and Ras signaling cascades. SYK Y352 phosphorylation by Fyn/Lyn is critical for propagation of B cell receptor (BCR) signaling and for B cell development.

          Specificity of this n3kobu5 clone was determined by ELISA, flow cytometry, and western blotting.

          Applications Reported:This n3kobu5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This n3kobu5 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

          Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          ZAP70 and SYK are closely related cytoplasmic tyrosine kinases that play central roles in immune receptor signaling. ZAP70 (Zeta-chain-associated protein kinase 70; gene: ZAP70) is primarily expressed in T lymphocytes and natural killer (NK) cells, where it is recruited to the T cell receptor (TCR) complex following antigen recognition. Upon activation, ZAP70 phosphorylates downstream adaptor proteins and initiates signaling cascades that drive T cell activation, proliferation, and differentiation. SYK (Spleen Tyrosine Kinase; gene: SYK) is predominantly expressed in B cells and other hematopoietic cells and functions analogously in B cell receptor (BCR) signaling, as well as in Fc receptor and integrin-mediated pathways. Although ZAP70 and SYK share structural homology and overlapping signaling mechanisms, they exhibit distinct expression patterns and immunological roles. Dysregulation of either kinase has been implicated in autoimmune disease, immunodeficiency, and hematologic malignancies, making them important targets in immunology and therapeutic research.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; p72-Syk; Spleen tyrosine kinase; Syk kinase; Syk-related kinase; Syk-related tyrosine kinase; TCR-associated protein tyrosine kinase; Tyrosine-protein kinase SYK; Tyrosine-protein kinase ZAP-70; unnamed protein product; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

          View more View less

          Gene Aliases: ADMIO2; IMD48; IMD82; mrtle; mur; p72-Syk; ptk72; SRK; STCD; STD; SYK; Sykb; TZK; ZAP-70; ZAP70

          View more View less

          UniProt ID: (Human) P43405, (Mouse) P48025, (Human) P43403, (Mouse) P43404

          View more View less

          Entrez Gene ID: (Human) 6850, (Mouse) 20963, (Human) 7535, (Mouse) 22637

          View more View less

          Function(s)
          nucleotide binding phosphotyrosine binding protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding integrin binding protein binding ATP binding interleukin-15 receptor binding kinase activity transferase activity protein kinase binding phosphatase binding Toll-like receptor binding SH2 domain binding phospholipase binding scaffold protein binding receptor signaling protein tyrosine kinase activity transferase activity, transferring phosphorus-containing groups protein domain specific binding kinase modulator
          Process(es)
          autophagosome assembly angiogenesis cell activation positive regulation of cytokine production serotonin secretion lymph vessel development positive regulation of receptor internalization stimulatory C-type lectin receptor signaling pathway adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response leukocyte activation involved in immune response immune system process serotonin secretion by platelet cell surface pattern recognition receptor signaling pathway negative regulation of inflammatory response to antigenic stimulus protein phosphorylation protein import into nucleus leukocyte cell-cell adhesion cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway integrin-mediated signaling pathway organ morphogenesis negative regulation of autophagy positive regulation of autophagy regulation of platelet activation regulation of tumor necrosis factor-mediated signaling pathway peptidyl-tyrosine phosphorylation leukotriene biosynthetic process calcium-mediated signaling platelet activation B cell differentiation neutrophil chemotaxis positive regulation of protein complex assembly receptor internalization cellular response to nutrient levels positive regulation of type I interferon production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-3 production positive regulation of interleukin-4 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of mast cell cytokine production regulation of superoxide anion generation positive regulation of superoxide anion generation positive regulation of cell adhesion mediated by integrin intracellular signal transduction collagen-activated tyrosine kinase receptor signaling pathway Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis interleukin-3-mediated signaling pathway TORC1 signaling gamma-delta T cell differentiation defense response to bacterium mast cell degranulation positive regulation of mast cell degranulation regulation of neutrophil degranulation beta selection positive regulation of MAPK cascade innate immune response positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of lymphocyte differentiation positive regulation of bone resorption positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation blood vessel morphogenesis regulation of phagocytosis regulation of immune response positive regulation of calcium-mediated signaling B cell receptor signaling pathway leukocyte migration regulation of sequence-specific DNA binding transcription factor activity establishment of localization in cell regulation of ERK1 and ERK2 cascade cellular response to molecule of fungal origin cellular response to lipid cellular response to low-density lipoprotein particle stimulus positive regulation of monocyte chemotactic protein-1 production regulation of arachidonic acid secretion regulation of platelet aggregation apoptotic signaling pathway beta-amyloid clearance positive regulation of cold-induced thermogenesis negative regulation of TORC1 signaling positive regulation of TORC1 signaling cellular response to beta-amyloid cellular response to lectin activation of MAPK activity transmembrane receptor protein tyrosine kinase signaling pathway G-protein coupled receptor signaling pathway activation of JUN kinase activity phosphorylation cell migration peptidyl-serine phosphorylation signal transduction by protein phosphorylation cell differentiation peptidyl-tyrosine autophosphorylation transcription factor import into nucleus positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process protein autophosphorylation positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation immune response T cell differentiation T cell activation B cell activation positive thymic T cell selection negative thymic T cell selection thymic T cell selection positive regulation of T cell differentiation alpha-beta T cell differentiation T cell receptor signaling pathway T cell aggregation T cell migration
          It has to be done as per old AB suggested Products section.

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