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Invitrogen

CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), PE, eBioscience™

2 Published Figures
9 References
View all (106) NGFR (CD271) antibodies

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Product Details

12-9400-41

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
View 8 publications 8 publications

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat

Published species

Dog, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ME20.4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572709

Product Specific Information

Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa, type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.

The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271.

Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, and microscopy.

Applications Tested: This ME20.4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated HEK-293T cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: CD271 antigen; gp80-LNGFR; low affinity nerve growth factor receptor; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor; low-affinity nerve growth factor receptor p75NGFR; low-affinity nerve growth factor receptor p75NGR; nerve growth factor receptor (TNFR superfamily, member 16); nerve growth factor receptor, fast; NGF receptor; NGFR; p-75; p75 ICD; p75 neurotrophin receptor; p75 ntr; TNFR superfamily, member 16; TNR16; unnamed protein product

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Gene Aliases: CD271; Gp80-LNGFR; LNGFR; NGR; p75; p75(NTR); p75NGFR; p75NTR; RNNGFRR; TNFRSF16

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UniProt ID: (Human) P08138, (Mouse) Q9Z0W1, (Rat) P07174

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Entrez Gene ID: (Pig) 100525607, (Human) 4804, (Sheep) 101119064, (Dog) 491071, (Rabbit) 100349095, (Mouse) 18053, (Rat) 24596

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Function(s)
beta-amyloid binding transmembrane signaling receptor activity death receptor activity protein binding calmodulin binding coreceptor activity small GTPase binding ubiquitin protein ligase binding signaling receptor activity neurotrophin binding nerve growth factor binding neurotrophin TRKA receptor binding identical protein binding macromolecular complex binding preprotein binding transmembrane signal receptor
Process(es)
cellular glucose homeostasis intracellular protein transport signal transduction Rho protein signal transduction circadian rhythm negative regulation of cell migration circadian regulation of gene expression dorsal aorta development regulation of cell proliferation glucose homeostasis positive regulation of apoptotic process neuron apoptotic process extrinsic apoptotic signaling pathway presynaptic modulation of chemical synaptic transmission positive regulation of protein localization to nucleus positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis cellular response to beta-amyloid positive regulation of endothelial cell apoptotic process axon guidance central nervous system development regulation of gene expression positive regulation of neuron projection development negative regulation of neuron projection development detection of temperature stimulus negative regulation of angiogenesis nerve development hair follicle morphogenesis positive regulation of myelination positive regulation of synaptic transmission, cholinergic cellular response to oxidative stress positive regulation of Rho protein signal transduction negative regulation of fibroblast growth factor receptor signaling pathway positive regulation of odontogenesis of dentin-containing tooth negative regulation of apoptotic process positive regulation of MAPK cascade negative regulation of neuron apoptotic process skin development positive regulation of neuron differentiation positive regulation of fibroblast proliferation negative regulation of multicellular organismal process negative regulation of hair follicle development positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization positive regulation of synaptic transmission, glutamatergic negative regulation of dendritic spine development positive regulation of neural precursor cell proliferation regulation of reactive oxygen species metabolic process positive regulation of excitatory postsynaptic potential positive regulation of apoptotic signaling pathway spermatogenesis response to lipopolysaccharide skeletal muscle cell differentiation response to ethanol
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