Advanced DMEM This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 12491023

Gibco™  Related applications: Mammalian Cell Culture

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Description

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

This Advanced DMEM is manufactured as follows:
WithWithout
• High glucose• L-glutamine
• Non Essential Amino Acids
• Sodium pyruvate
• Phenol Red

The complete formulation is available.

Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Advanced DMEM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
Glucose: High Glucose
Serum Supplementation: Advanced (Reduced Serum)
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Product Size: 10 x 500 mL
Volume (Metric): 500 ml
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media formulations

Documents

Manuals & protocols

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