Serum-free media for the growth of primary bovine myoblasts.
AuthorsKolkmann AM,Post MJ,Rutjens MAM,van Essen ALM,Moutsatsou P
JournalCytotechnology
PubMed ID31884572
The demand for meat is expected to exceed production capacity by livestock in the coming decennia. Therefore, cultured beef might be a viable alternative to traditional livestock-derived beef. One of the problems however is the sustainability of cultured beef through the use of fetal bovine serum. We aimed to identify ... More
Viral complementation allows HIV-1 replication without integration.
BACKGROUND: The integration of HIV-1 DNA into cellular chromatin is required for high levels of viral gene expression and for the production of new virions. However, the majority of HIV-1 DNA remains unintegrated and is generally considered a replicative dead-end. A limited amount of early gene expression from unintegrated DNA ... More
miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin.
AuthorsZhuang J,Fan J,Zhu L,Zhao L,Huang Y,Pan X,Guo T
JournalJournal of Cancer
PubMed ID37497401
Background: Non-small cell lung cancer (NSCLC) is a common malignant tumor, and it is characterized by high mortality. MicroRNA-452-5p (miR-452-5p) and Moesin (MSN) have been proved to be related with regulation of tumors. If miR-452-5p could regulate NSCLC through targeting MSN remain unclear. Methods: TargetScan data and GEPIA databases were ... More
Down-regulation of miR-424 inhibited the metastasis of endometrial carcinoma via targeting PTEN/PI3K/AKT signaling pathway.
AuthorsLu Y,Lin Q,Lin C,Chen J,Jiang X,He H
JournalJournal of Cancer
PubMed ID37781075
Background: The incidence of endometrial carcinoma (EC) has been increasing annually, and treatment of advanced cases remains challenging. MicroRNA-424 (miR-424) was reported to affect several types of tumors, but its role in EC has not been studied. Methods: We generated transient knockdown models of miR-424 and PTEN in EC cells. ... More
An in vivo model of human small intestine using pluripotent stem cells.
Differentiation of human pluripotent stem cells (hPSCs) into organ-specific subtypes offers an exciting avenue for the study of embryonic development and disease processes, for pharmacologic studies and as a potential resource for therapeutic transplant(1,2). To date, limited in vivo models exist for human intestine, all of which are dependent upon ... More
Understanding factors affecting human chondrocyte culturing: an experimental study.
AuthorsKilic P,Gurcan C,Gurman G,Yilmazer A
JournalCell and tissue banking
PubMed ID32671509
Over the years, surgical strategies have been developed in hope of full regeneration of the injured cartilage. In our study, we aimed to develop an optimized chondrocyte culture isolation technique as an active ingredient of a standardized autologous chondrocte implantation product, which is able to maintain the phenotype along with ... More
The Isolation of Human Glioblastoma Cells: An Optimised Protocol.
AuthorsVelnar T,Maver U,Bosnjak R,Gradisnik L
JournalActa medica academica
PubMed ID33543625
Objective: The aim of this study was to establish an optimised protocol for glioblastoma (GBM) cell isolation from brain resection samples, with a high yield and low risk for contamination. Methods: Human GBM cells can be obtained following cranial tumour operations. In sterile conditions, the fragments of viable tissue removed ... More
Effects of curcumin complexes on MDA‑MB‑231 breast cancer cell proliferation.
AuthorsMohammed F,Rashid-Doubell F,Taha S,Cassidy S,Fredericks S
JournalInternational journal of oncology
PubMed ID32626932
Curcumin displays anticancer properties; however, some issues with the drug delivery mode limit its therapeutic use. Although reformulation and derivatization of curcumin have improved its bioavailability, curcumin derivatives may not retain the same anticancer properties as the parent compound. The present study investigated the anticancer properties of two curcumin complexes, ... More
Surface functionalization of carbon nanomaterials by self-assembling hydrophobin proteins.
AuthorsYang W, Ren Q, Wu YN, Morris VK, Rey AA, Braet F, Kwan AH, Sunde M,
JournalBiopolymers
PubMed ID23097233
'Class I fungal hydrophobins are small surface-active proteins that self-assemble to form amphipathic monolayers composed of amyloid-like rodlets. The monolayers are extremely robust and can adsorb onto both hydrophobic and hydrophilic surfaces to reverse their wettability. This adherence is particularly strong for hydrophobic materials. In this report, we show that ... More
Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.
AuthorsVacanti V, Kong E, Suzuki G, Sato K, Canty JM, Lee T,
JournalJ Cell Physiol
PubMed ID15880640
'The in vitro culture of porcine bone marrow-derived mesenchymal stem cells (MSCs) was used for the investigation of adult stem cell biology. Isolated porcine MSCs possessed the ability to proliferate extensively in Gibco(R) Advanced DMEM containing 5% fetal bovine serum (FBS). Greater than 40 serial MSC passages and 100 cell ... More