SUMO Protease - FAQs

View additional product information for SUMO Protease - FAQs (12588018)

4 product FAQs found

Are NP40 and DTT required for SUMO Protease activity? Will they interfere with downstream applications?

Yes, they are both required, although it might be possible to substitute the NP40 with another detergent via dialysis if you have concern about interference with mass spectrometry. DTT is an absolute requirement.

What enzyme should I choose if I do not want any extra amino acids left on my protein of interest after cleavage?

We would recommend using our SUMO system.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does SUMO protease work?

SUMO protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence. The SUMO protease itself has a His tag for easy removal from the protein mix after cleavage.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What products do you offer for enzymatic cleavage of fusion tags from recombinant proteins?

We offer the following products:

-AcTEV Protease (Cat. Nos. 12575015, 12575023)
-EKMax Enterokinase (Cat. Nos. E18001, E18002)
-SUMO Protease (Cat. No. 12588018)

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.