Invitrogen™

SUMO Protease

Catalog number:  12588018

Related applications:

Protein Biology

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Description

SUMO Protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence. The SUMO Protease features:

• Highly active cleavage
• No non-specific proteolysis (highly specific cleavage*)
• Activity from 2°C to 37°C
• A six-histidine sequence to facilitate its removal from the digested protein sample

Application: Removal of fusion tags from recombinant proteins.
Unit Definition: One unit of SUMO Protease is defined as the amount of enzyme needed to cleave 85% of 2µg of substrate protein at 30°C in one hour.
Quality Control: SUMO Protease has greater than 85% single-band purity with no non-specific protease contamination. It is functionally tested for the absence of any non-specific protease activity.
5 enzymes
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition: Dry Ice
Species: S. cerevisiae

Contents & storage

SUMO Protease is provided with 10X high salt and 10X no salt dilution buffers. Store at -80°C. Guaranteed stable for 6 months when properly stored.