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CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Biotin, eBioscience™
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-Flow-20170216130641.jpg?time=20240507 "CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 64
CD274 (PD-L1, B7-H1) Antibody (13-5982-81) in Flow
Staining of C57Bl/6 splenocytes with 0.125 µg of Rat IgG2a Isotype Control Biotin (Product # 13-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD274 (B7-H1) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
-Flow-20170216130641.jpg?time=20240507)
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD274 (PD-L1, B7-H1) Antibody (13-5982-81) in Flow")
Product Details
13-5982-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, lambda
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MIH5
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.
Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B7 homolog 1; PDCD1 ligand 1; pdl-1; sCD274; soluble CD274; soluble PD L1; sPD L1; unnamed protein product
Gene Aliases: A530045L16Rik; B7h1; Pdcd1l1; Pdcd1lg1; Pdl1
UniProt ID: (Mouse) Q9EP73
Entrez Gene ID: (Mouse) 60533
negative regulation of T cell mediated immune response to tumor cell
immune response
signal transduction
cell surface receptor signaling pathway
positive regulation of cell migration
T cell costimulation
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
positive regulation of interleukin-10 production
response to cytokine
TRIF-dependent toll-like receptor signaling pathway
positive regulation of T cell proliferation
negative regulation of T cell proliferation
positive regulation of transcription, DNA-templated
negative regulation of activated T cell proliferation
negative regulation of T cell receptor signaling pathway
negative regulation of T cell activation
cellular response to lipopolysaccharide
negative regulation of tumor necrosis factor superfamily cytokine production
positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process
negative regulation of CD4-positive, alpha-beta T cell proliferation
negative regulation of CD8-positive, alpha-beta T cell activation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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