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Invitrogen
CD338 (ABCG2) Monoclonal Antibody (5D3), Biotin, eBioscience™
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FIGURE: 1 / 15
CD338 (ABCG2) Antibody (13-8888-82) in Flow
Staining of MCF/huBCRP-1 transfected cells with 0.5 µg of Anti-Mouse IgG2b Biotin (Product # 13-4732-85) (open histogram) or 0.5 µg of Anti-Human CD338 (ABCG2) Biotin followed by Streptavidin PE (Product # 12-4317-87) (filled histogram). Total viable cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-8888-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Virus
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
5D3
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 5D3 monoclonal antibody reacts with the extracellular portion of the human ABCG2 protein, also known as Bcrp1 and MXR. The ABCG2 gene, a member of the multi-drug resistance (MDR) family, is highly expressed on primitive 'side-population' (SP) stem cells, which are defined by the efflux of fluorescent dyes such as Rhodamine 123 and Hoechest 33342. In the bone marrow, about 0.05% of cells display the low fluorescence and are highly enriched for repopulating cells. The SP cells, which express low or undetectable levels of CD34, have been identified in multiple species. In addition, expression of ABCG2 appears to be highly conserved.
Applications Reported: The 5D3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 5D3 antibody has been tested by flow cytometric analysis of human ABCG2 transfected cells and normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ABCG2, is also known as placenta-specific ABC transporter and breast cancer resistance protein (BCRP1). ABCG2 confers resistance for a variety of chemotherapeutic agents, including anthracyclines, mitoxantrone, bisantrene and topotecan. Under normal conditions, ABCG2 may serve a protective function by removing toxins from the cell, and plays an important role in regulating stem cell differentiation. ABCG2 is responsible for the side population (SP) phenotype and is widely expressed in a large variety of stem cells, making it an important stem cell marker. ABCG2 may have N-linked glycosylation and may dimerize in vivo. ABCG2 is abundantly expressed in placenta, liver, intestine and stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABC transporter; ABCG2; ATP binding cassette subfamily G member 2 (Junior blood group); ATP-binding cassette transporter; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group); ATPbinding cassette transporter ABCG2; breast cancer resistance protein; mitoxantrone resistance-associated protein; multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2; placenta specific MDR protein; placenta-specific ATP-binding cassette transporter; transporter; unnamed protein product; urate exporter
Gene Aliases: ABC15; ABCP; BCRP; BMDP; CD338; CDw338; CDw388; EST157481; GOUT1; MRX; MXR; MXR-1; MXR1; UAQTL1
UniProt ID: (Human) Q9UNQ0
Entrez Gene ID: (Human) 9429
protein binding
ATP binding
xenobiotic-transporting ATPase activity
urate transmembrane transporter activity
biotin transporter activity
efflux transmembrane transporter activity
ATPase activity
transmembrane transporter activity
riboflavin transporter activity
ATPase activity, coupled to transmembrane movement of substances
identical protein binding
protein homodimerization activity
xenobiotic transporter activity
protein dimerization activity
ABC-type transporter activity
lipid transport
urate transport
biotin transport
sphingolipid biosynthetic process
riboflavin transport
xenobiotic transport
urate metabolic process
transmembrane transport
transepithelial transport
urate salt excretion
detoxification
export across plasma membrane
transport across blood-brain barrier
sulfite export across plasma membrane
cellular detoxification
drug transmembrane export
drug transport across blood-brain barrier
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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