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Invitrogen
GFAP Monoclonal Antibody (2.2B10)
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FIGURE: 1 / 96
GFAP Antibody (13-0300) in ICC/IF
Immunofluorescent analysis of beta-3 Tubulin (green) and GFAP (red) in human iPSC-derived forebrain organoids derived at Day 84. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse beta-3 Tubulin monoclonal antibody (green; Produ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-0300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Avian,
Cat,
Cynomolgus monkey,
Dog,
Ferret,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Rat,
Rhesus monkey
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
2.2B10
Immunogen
Enriched bovine glial filaments.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein
Gene Aliases: AI836096; GFAP
UniProt ID: (Bovine) Q28115, (Rat) P47819, (Mouse) P03995
Entrez Gene ID: (Bovine) 281189, (Rat) 24387, (Mouse) 14580
intermediate filament organization
response to wounding
positive regulation of Schwann cell proliferation
negative regulation of neuron projection development
astrocyte development
extracellular matrix organization
neuron projection regeneration
intermediate filament-based process
regulation of neurotransmitter uptake
Bergmann glial cell differentiation
positive regulation of glial cell proliferation
long-term synaptic potentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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