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Invitrogen
NEFM Monoclonal Antibody (RMO-44)
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FIGURE: 1 / 5
NEFM Antibody (13-0500) in IHC (P)
Immunofluorescent analysis of the neurofilament medium chain in paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues... View More
Product Details
13-0500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Leech,
Rat
Published species
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
RMO-44
Immunogen
Rat neurofilaments
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
purified
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 160 kDa neurofilament protein; NEFM; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament triplet M protein; 160 KD neurofilament protein, NF-M; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NF-M; NMC; unnamed protein product
Gene Aliases: NEF3; NEFM; NF-M; NFM
UniProt ID: (Human) P07197, (Rat) P12839
Entrez Gene ID: (Human) 4741, (Rat) 24588
neurofilament bundle assembly
microtubule cytoskeleton organization
axo-dendritic transport
spinal cord development
axon regeneration
regulation of axon diameter
cellular response to oxidative stress
intermediate filament cytoskeleton organization
intermediate filament polymerization or depolymerization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
cellular response to estradiol stimulus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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