Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells.
AuthorsChoi KD, Vodyanik M, Slukvin II,
JournalNat Protoc
PubMed ID21372811
'In this paper, we describe a protocol for hematopoietic differentiation of human pluripotent stem cells (hPSCs) and generation of mature myeloid cells from hPSCs through expansion and differentiation of hPSC-derived lin(-)CD34(+)CD43(+)CD45(+) multipotent progenitors. The protocol comprises three major steps: (i) induction of hematopoietic differentiation by coculture of hPSCs with OP9 ... More
Long term non-invasive imaging of embryonic stem cells using reporter genes.
AuthorsSun N, Lee A, Wu JC,
JournalNat Protoc
PubMed ID19617890
'Development of non-invasive and accurate methods to track cell fate after delivery will greatly expedite transition of embryonic stem (ES) cell therapy to the clinic. In this protocol, we describe the in vivo monitoring of stem cell survival, proliferation and migration using reporter genes. We established stable ES cell lines ... More
Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice.
Authors Marron Michele P; Graser Robert T; Chapman Harold D; Serreze David V;
JournalProc Natl Acad Sci U S A
PubMed ID12361980
Particular major histocompatibility complex (MHC) class II alleles clearly contribute to T cell-mediated autoimmune type 1 diabetes (T1D) in both humans and nonobese diabetic (NOD) mice. However, studies in NOD mice indicate MHC class I-restricted T cell responses are also essential to T1D development. In humans, epidemiological studies have suggested ... More