Zitierungen und Referenzen (3)

Gibco™

Zelldissoziationspuffer, enzymfrei, PBS

Der Gibco™ Zelldissoziationspuffer ist eine durch eine Membran gefilterte, isotonische und enzymfreie Lösung aus Salzen, Chelatbildnern und Stoffen zur ZellkonditionierungWeitere Informationen

Katalognummer	Menge
13151014	100 ml

Katalognummer 13151014

Preis (EUR)

56,50

Menge:

100 ml

Preis (EUR)

56,50

Der Gibco™ Zelldissoziationspuffer ist eine durch eine Membran gefilterte, isotonische und enzymfreie Lösung aus Salzen, Chelatbildnern und Stoffen zur Zellkonditionierung in einer Phosphat-gepufferten Kochsalzlösung (PBS), die kein Calcium und Magnesium enthält. Der Gibco™ Zelldissoziationspuffer ist für die sanfte Dissoziation von Säugerzellen für Studien wie Ligandenbindung, Durchflusszytometrie und Immunhistochemie geeignet, bei denen intakte Zelloberflächenproteine benötigt werden. Der Gibco™ Zelldissoziationspuffer funktioniert gut mit leicht anhaftenden Zelllinien, z. B. HeLa und NIH 3T3, wird aber nicht für die routinemäßige Passage stark adhärenter Zellen empfohlen. Das Dissoziationsprotokoll ist in unserer Technical Reference Library verfügbar.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZelllinieHeLa, NIH 3T3

ChelatbildnerEDTA

Menge100 ml

BemerkungenStabil bei Raumtemperatur

Haltbarkeit24 Monate

VersandbedingungRaumtemperatur

KlassifikationOhne Stoffe tierischen Ursprungs

ProdukttypReagenz zur Zellkulturdissoziation

SterilitätSteril gefiltert

Mit AdditivenEDTA

Unit SizeEach

Inhalt und Lagerung

Lagerbedingungen: 15 bis 30 °C
Versandbedingungen: Haltbarkeit bei
Raumtemperatur: 24 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

What is the osmolality and pH range for Cell Dissociation Buffer, enzyme-free, PBS (Cat. No. 13151014)

Below are QC test specifications for pH and osmolality for this product:

- pH: 6.9 to 7.9
- Osmolality: 289 to 321 mOsm/kg

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

At what confluency should cells be at prior to their dissociation using the Cell Association Buffer, enzyme-free, PBS?

The buffer should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, it will be hard to detach them.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the Cell Dissociation Buffer, enzyme-free, PBS damage cell surface markers/receptors?

This buffer is very gentle. It should preserve the structural and functional integrity of cell surface proteins. However, we recommend that the customer test their cells and proteins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use the Cell Dissociation Buffer, enzyme-free, PBS to dissociate cells?

This Enzyme-Free Cell Dissociation Buffer does not rely on proteolysis for its action. Therefore, it is not suitable for strongly adherent cells, or for routine passage of some cell types (e.g., A431, Hep2, A549, MDCK, WI-38). This product is intended to gently dissociate cells from attachment substrates and each other while maintaining surface protein integrity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Zitierungen und Referenzen (3)

Zitierungen und Referenzen

Abstract

Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells.

Authors:Choi KD, Vodyanik M, Slukvin II,

Journal:Nat Protoc

PubMed ID:21372811

'In this paper, we describe a protocol for hematopoietic differentiation of human pluripotent stem cells (hPSCs) and generation of mature myeloid cells from hPSCs through expansion and differentiation of hPSC-derived lin(-)CD34(+)CD43(+)CD45(+) multipotent progenitors. The protocol comprises three major steps: (i) induction of hematopoietic differentiation by coculture of hPSCs with OP9 ... More

Long term non-invasive imaging of embryonic stem cells using reporter genes.

Authors:Sun N, Lee A, Wu JC,

Journal:Nat Protoc

PubMed ID:19617890

'Development of non-invasive and accurate methods to track cell fate after delivery will greatly expedite transition of embryonic stem (ES) cell therapy to the clinic. In this protocol, we describe the in vivo monitoring of stem cell survival, proliferation and migration using reporter genes. We established stable ES cell lines ... More

Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice.

Authors: Marron Michele P; Graser Robert T; Chapman Harold D; Serreze David V;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12361980

Particular major histocompatibility complex (MHC) class II alleles clearly contribute to T cell-mediated autoimmune type 1 diabetes (T1D) in both humans and nonobese diabetic (NOD) mice. However, studies in NOD mice indicate MHC class I-restricted T cell responses are also essential to T1D development. In humans, epidemiological studies have suggested ... More