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ERK1/ERK2 Monoclonal Antibody (ERK-7D8)
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FIGURE: 1 / 31
ERK1/ERK2 Antibody (13-6200) in ICC/IF
Immunofluorescent analysis of ERK1 + ERK2 Antibody (ERK-7D8) was done on 70% confluent log phase U87-MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ERK1 + ERK2 Antibody (ERK-7D8) (Product # 13-6200) at 1:250 dilution in1% BSA and incubated for 3 ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-6200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Hamster,
Human,
Mouse,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ERK-7D8
Immunogen
Synthetic peptide (aa 324-345) corresponding to a segment near the C-terminus of rat ERK1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
200 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts strongly with both ERK1 and ERK2. Reactivity has been confirmed by western blot analysis of extracts derived from human A431 and K562 cells, canine MDCK cells, mouse brain, rat brain, and recombinant ERK2.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ERK-2; erk1 erk2; Erk1; cancer susceptibility protein; ERK1; MAP kinase; mitogen-activated protein kinase; ERK1b; ERK1c; Erk2; cancer susceptibility protein; ERT1; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAPK 1; MAPK 2; MAPK 3; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; pp42/MAP kinase; protein serine/threonine kinase ERK1b; protein tyrosine kinase ERK2; unnamed protein product
Gene Aliases: 9030612K14Rik; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MAPK2; MNK1; Mtap2k; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3
UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708
Entrez Gene ID: (Human) 5594, (Dog) 477575, (Dog) 100855877, (Human) 5595, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689
phosphotyrosine binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
phosphatase binding
identical protein binding
protein serine kinase activity
DNA-binding transcription factor binding
double-stranded DNA binding
protein kinase binding
mitogen-activated protein kinase kinase kinase binding
scaffold protein binding
non-receptor serine/threonine protein kinase
MAPK cascade
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
apoptotic process
chemotaxis
signal transduction
cell surface receptor signaling pathway
epidermal growth factor receptor signaling pathway
chemical synaptic transmission
learning or memory
insulin receptor signaling pathway
positive regulation of macrophage chemotaxis
positive regulation of peptidyl-threonine phosphorylation
Schwann cell development
positive regulation of telomere maintenance
regulation of stress-activated MAPK cascade
cellular response to amino acid starvation
response to nicotine
intracellular signal transduction
ERBB signaling pathway
ERBB2-ERBB3 signaling pathway
myelination
positive regulation of skeletal muscle tissue regeneration
positive regulation of cholesterol biosynthetic process
positive regulation of smoothened signaling pathway
insulin-like growth factor receptor signaling pathway
stress-activated MAPK cascade
regulation of cytoskeleton organization
interleukin-34-mediated signaling pathway
chemokine-mediated signaling pathway
ERK1 and ERK2 cascade
response to epidermal growth factor
caveolin-mediated endocytosis
regulation of Golgi inheritance
positive regulation of macrophage proliferation
positive regulation of neuroinflammatory response
regulation of early endosome to late endosome transport
negative regulation of T cell mediated immune response to tumor cell
phosphorylation
sensory perception of pain
BMP signaling pathway
positive regulation of cyclase activity
peptidyl-tyrosine autophosphorylation
signal transduction in response to DNA damage
positive regulation of transcription from RNA polymerase II promoter
modulation of synaptic transmission
positive regulation of ERK1 and ERK2 cascade
interleukin-1-mediated signaling pathway
cellular response to mechanical stimulus
negative regulation of cholesterol efflux
negative regulation of TORC1 signaling
cellular response to DNA damage stimulus
heart development
positive regulation of cell proliferation
response to toxic substance
organ morphogenesis
neural crest cell development
diadenosine tetraphosphate biosynthetic process
peptidyl-serine phosphorylation
cytosine metabolic process
regulation of ossification
positive regulation of cell migration
thyroid gland development
lipopolysaccharide-mediated signaling pathway
response to estradiol
response to lipopolysaccharide
response to testosterone
mammary gland epithelial cell proliferation
cellular response to platelet-derived growth factor stimulus
response to cocaine
positive regulation of protein import into nucleus
outer ear morphogenesis
response to hydrogen peroxide
response to exogenous dsRNA
response to estrogen
estrous cycle
negative regulation of cell differentiation
positive regulation of translation
positive regulation of transcription, DNA-templated
decidualization
thymus development
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of intracellular pH
Bergmann glial cell differentiation
positive regulation of cardiac muscle cell proliferation
long-term synaptic potentiation
face development
lung morphogenesis
trachea formation
labyrinthine layer blood vessel development
cardiac neural crest cell development involved in heart development
cellular response to methionine
cellular response to cAMP
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
cellular response to peptide hormone stimulus
cellular response to prostaglandin E stimulus
cellular response to lipid
cellular response to toxic substance
response to alcohol
cellular response to insulin-like growth factor stimulus
placenta development
autophagosome assembly
negative regulation of autophagy
positive regulation of autophagy
cellular response to nutrient levels
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
TORC1 signaling
protein stabilization
negative regulation of T cell activation
cartilage development
protein-containing complex assembly
positive regulation of xenophagy
arachidonic acid metabolic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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