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Antibody Testing Data (1)

CD100 (SEMA4D) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

CD100 (SEMA4D) Antibody (14-1001-37) in Flow

Staining of C57BL/6 splenocytes with 0.125 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD100 (SEMA4D) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Product Details

14-1001-37

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

Flow Cytometry (Flow)

0.25 µg/test

Immunoprecipitation (IP)

Assay-Dependent

Functional Assay (Functional)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BMA12 (BMA-12)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

2 mg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Ambient (domestic); Wet ice (international)

AB_2864950

Product Specific Information

Description: The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D. Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72. Interaction of these two proteins diminishes the negative signaling normally effected by CD72. CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types. Antigen expression has been reported in Spleen, thymus and lymph nodes.

Applications Reported: The BMA12 (BMA-12) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported for use in neutralization of mouse CD100. (Please use Functional Grade purified BMA12, Product # 16-1001, in functional assays).

Applications Tested: The BMA12 (BMA-12) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: M-sema G; sema J; semaphorin H; semaphorin-C-like 2; semaphorin-J; unnamed protein product

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Gene Aliases: CD100; coll-4; Semacl2; Semaj; Semcl2

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UniProt ID: (Mouse) O09126

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Entrez Gene ID: (Mouse) 20354

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Function(s)

transmembrane signaling receptor activity receptor binding protein binding semaphorin receptor binding signaling receptor activity neuropilin binding identical protein binding chemorepellent activity receptor agonist activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD100 (SEMA4D) Monoclonal Antibody (BMA12 (BMA-12)), eBioscience™

CD100 (SEMA4D) Antibody (14-1001-37) in Flow

Product Details

14-1001-37

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (Functional)

Species Reactivity

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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