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Invitrogen
EOMES Monoclonal Antibody (21Mags8), eBioscience™
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-4876-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
21Mags8
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 21Mags8 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.
Note: For detection of mouse Eomes, we recommend 12-4875 and 51-4875 and for detection of human Eomes, we recommend 51-4877 due to the brighter staining intensity of these products compared to clone 21Mags8.
Applications Reported: This 21Mags8 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).
Applications Tested: This 21Mags8 antibody has been tested by western blot analysis of mouse splenocytes. As a starting concentration, this antibody can be used at 2 µg/mL.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: T-box brain protein 2; T-box family member; T-box domain; T-brain-2; unnamed protein product
Gene Aliases: TBR-2; TBR2
UniProt ID: (Human) O95936, (Mouse) O54839
Entrez Gene ID: (Human) 8320, (Mouse) 13813
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
chromatin DNA binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
negative regulation of transcription from RNA polymerase II promoter
endoderm formation
mesoderm formation
endodermal cell fate specification
neuron migration
CD8-positive, alpha-beta T cell differentiation involved in immune response
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
brain development
gene expression
cerebral cortex neuron differentiation
cerebral cortex development
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
astrocyte differentiation
cell differentiation involved in embryonic placenta development
mesodermal to mesenchymal transition involved in gastrulation
blastocyst development
trophectodermal cell differentiation
cardioblast differentiation
regulation of gene expression
stem cell population maintenance
olfactory bulb development
cerebral cortex regionalization
forebrain neuron differentiation
neurogenesis
cell differentiation
neuron differentiation
skeletal muscle cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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