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Invitrogen
Myogenin Monoclonal Antibody (F5D), eBioscience™
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FIGURE: 1 / 17
Myogenin Antibody (14-5643-37) in ICC/IF
Immunofluorescence analysis of Myogenin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myogenin Mouse Monoclonal Antibody (F5D) (Product # 14-5643-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-5643-37
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Dog,
Human,
Mouse,
Reptile
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
F5D
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
2 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This F5D monoclonal antibody reacts with human, mouse, dog, and rat myogenin, a 34-kDa transcription factor. Expressed in skeletal and heart muscle, myogenin is a member of the MyoD family of basic-helix-loop-helix proteins, which also includes MyoD, Myf5, and MRF4. This transcription factor interacts with other helix-loop-helix proteins, which may or may not be muscle-specific. Myogenin plays a significant role in myogenic differentiation, even directing nonmuscle cells to the myogenic lineage. Transforming growth factor-beta (TGFb) and bone morphogenetic protein-2 (BMP2) inhibit myogenin transcriptional activity. Predominantly residing within the nucleus, the subcellular localization of myogenin has been shown to be dependent on differentiation status and cell density. For instance, trafficking of myogenin between the nucleus and cytoplasm has been reported during skeletal muscle differentiation to mediate transcription control.
Applications Reported: This F5D antibody has been reported for use in immunoblotting (WB) and immunocytochemistry.
Applications Tested: This F5D antibody has been tested by western blot and immunocytochemistry of the C2C12 cell line. This can be used at less than or equal to 1-5 µg/mL for both applications. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: class C basic helix-loop-helix protein 3; MYOD1-related protein; myogenic factor 4; Myogenic factor-4; myogenic regulatory transcription factor; myogenin; unnamed protein product
Gene Aliases: bHLHc3; myf-4; MYF4; myo
UniProt ID: (Human) P15173, (Rat) P20428, (Mouse) P12979
Entrez Gene ID: (Human) 4656, (Dog) 490224, (Rat) 29148, (Mouse) 17928
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
chromatin DNA binding
sequence-specific DNA binding
protein dimerization activity
E-box binding
sequence-specific double-stranded DNA binding
basic helix-loop-helix transcription factor
regulation of transcription, DNA-templated
muscle organ development
skeletal muscle tissue development
negative regulation of cell proliferation
tissue development
positive regulation of myotube differentiation
positive regulation of muscle atrophy
regulation of skeletal muscle satellite cell proliferation
response to muscle activity involved in regulation of muscle adaptation
response to electrical stimulus involved in regulation of muscle adaptation
striated muscle atrophy
response to denervation involved in regulation of muscle adaptation
skeletal muscle cell differentiation
muscle cell differentiation
muscle cell fate commitment
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
animal organ development
positive regulation of skeletal muscle fiber development
regulation of cell cycle
cellular response to estradiol stimulus
regulation of myoblast fusion
ossification
response to gravity
skeletal muscle atrophy
response to muscle activity
myotube differentiation
skeletal muscle tissue regeneration
myoblast differentiation
negative regulation of glycolytic process
positive regulation of transcription, DNA-templated
skeletal muscle fiber development
cellular response to magnetism
cellular response to lithium ion
cellular response to retinoic acid
cellular response to tumor necrosis factor
cellular response to growth factor stimulus
positive regulation of oxidative phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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