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Invitrogen
Integrin beta 7 Monoclonal Antibody (FIB504), eBioscience™
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FIGURE: 1 / 2
Integrin beta 7 Antibody (14-5867-37) in Flow
Staining of normal human peripheral blood cells with 0.06 µg of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.06 µg of Anti-Human/Mouse Integrin β7 Purified (filled histogram) followed by F (ab')2 Anti-Rat IgG PE (Product # 12-4822).Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-5867-37
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
FIB504
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
2 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The FIB504 monoclonal antibody reacts with human and mouse integrin beta 7. Integrin beta 7 is a 130 kD glycoprotein which associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Main ligands for integrin alpha 4 beta 7 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the main ligand of integrin alpha E beta 7 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response. The FIB504 antibody has been reported to have blocking activity.
Applications Reported: This FIB504 antibody has been reported for use in flow cytometric analysis and functional assays.
Applications Tested: This FIB504 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: gut homing receptor beta subunit; integrin beta 7 subunit; integrin beta-P; lymphocyte Peyer's patch high endothelial venule adhesion molecule 1 homolog; M290 IEL antigen; unnamed protein product
Gene Aliases: Ly69
UniProt ID: (Human) P26010, (Mouse) P26011
Entrez Gene ID: (Human) 3695, (Mouse) 16421
immune response in gut-associated lymphoid tissue
cell-matrix adhesion involved in ameboidal cell migration
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
cell adhesion mediated by integrin
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
receptor clustering
leukocyte migration
leukocyte tethering or rolling
cell-cell adhesion
T cell migration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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