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Invitrogen
CD366 (TIM3) Monoclonal Antibody (8B.2C12), eBioscience™
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FIGURE: 1 / 5
CD366 (TIM3) Antibody (14-5871-81) in Flow
Staining of non-transfected (left) and mouse CD366-transfected (right) CHO cells with 0.125 µg of Rat IgG1 kappa Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.125 µg of Anti-Mouse CD366 (TIM3) Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85).Total cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-5871-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
8B.2C12
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 8B.2C12 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. CD366 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. CD366 is expressed selectively by differentiated CD4+ Th1 and CD8+ Tc1, but is absent on Th2 and Tc2. Other hematopoietic cell types, including naive T cells, B cells, macrophages and dendritic cells, do not express CD366, at least at the protein level. Expression of CD366 is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation. In an experimental autoimmune encephalomyelitis (EAE) model, CD366 was shown to be expressed on most CD4+ and CD8+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4+ cells in the periphery expressed CD366 after immunization. In this model, in vivo administration of 8B.2C12 resulted in a hyperacute and atypical disease phenotype. It is postulated that the engagement of CD366 during T cell activation results in the expansion and activation of macrophages and increased severity of autoimmune disease. The Tim gene family may have an important role in the regulation of autoimmunity and allergies.
The 8B.2C12 antibody binds to the BALB/c allele of CD366 while reactivity to the C57Bl/6 allele is significantly weaker.
Applications Reported: The 8B.2C12 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported for use in functional assays. (Please use Functional Grade purified 8B.2C12, Product # 16-5871-85, in functional assays).
Applications Tested: The 8B.2C12 antibody has been tested by flow cytometric analysis of mouse splenocytes and mouse CD366 transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ14428; HAVcr-2; sCD366; soluble CD366; soluble TIM 3; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM-3; TIMD-3; transmembrane protein; unnamed protein product
Gene Aliases: TIM-3; Tim3; Timd3
UniProt ID: (Mouse) Q8VIM0
Entrez Gene ID: (Mouse) 171285
positive regulation of cytokine production
macrophage activation involved in immune response
natural killer cell tolerance induction
regulation of tolerance induction dependent upon immune response
negative regulation of T-helper 1 type immune response
negative regulation of immune response to tumor cell
negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
regulation of transcription from RNA polymerase II promoter
negative regulation of gene expression
negative regulation of myeloid dendritic cell activation
negative regulation of type I interferon production
negative regulation of interferon-alpha production
negative regulation of interferon-gamma production
negative regulation of interleukin-2 production
negative regulation of interleukin-3 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of chemokine production
positive regulation of interferon-gamma production
positive regulation of interleukin-1 production
positive regulation of interleukin-4 production
positive regulation of tumor necrosis factor production
negative regulation of natural killer cell activation
toll-like receptor 3 signaling pathway
toll-like receptor 7 signaling pathway
toll-like receptor 9 signaling pathway
positive regulation of T cell proliferation
negative regulation of T cell proliferation
positive regulation of macrophage activation
positive regulation of innate immune response
negative regulation of innate immune response
defense response to Gram-positive bacterium
maternal process involved in female pregnancy
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
negative regulation of granulocyte colony-stimulating factor production
negative regulation of defense response to bacterium
positive regulation of defense response to bacterium
positive regulation of NIK/NF-kappaB signaling
negative regulation of immunological synapse formation
negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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