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Invitrogen
CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, eBioscience™
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FIGURE: 1 / 9
CXCL12 alpha (SDF-1 alpha) Antibody (14-7992-81) in IHC
Journal of cellular and molecular medicine 2020 -
Tfh cells are an important source of CXCL12 in mice with schistosomiasis japonica. WT and icosl -/- mice were infected with 12 cercariae of Schistosoma japonicum per mouse, and sacrificed at 8 wk post-infection. (A, B) Spleens, mesenteric LN and livers from normal and infected mice were harvested, and cells were stained with CD3-percpcy5.5, CD4-PE-Cy7, CXCR5-FITC, PD-1-PE and CXCL12-APC antibodies. CXCL12 +... View More
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20240507?time=20240507 "CXCL12 alpha (SDF-1 alpha) Antibody (14-7992-81) in IHC")
.jpg?time=20240507?time=20240507 "CXCL12 alpha (SDF-1 alpha) Antibody (14-7992-81) in IHC")
.jpg?time=20240507?time=20240507 "CXCL12 alpha (SDF-1 alpha) Antibody (14-7992-81) in WB, ELISA")
Product Details
14-7992-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The polyclonal antibody reacts with both mouse and rat SDF-1 alpha. It was was generated using E. coli-expressed N terminal amio acids 20-89 of mouse SDF-1 alpha as an immunogen. SDF-1 alpha (stromal cell-derived factor-1 alpha), also known as pre-B-cell growth-stimulating factor (PBSF), is a 70-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of the SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.
Applications Reported: This polyclonal has been reported for use in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to SDF-1 alpha of other species has not been determined.
Applications Tested: To achieve best results, titrate antibody by dilution for each application as follows:
Western Blot 1:1000
Immunoprecipitation 1:300-500
Immunohistochemistry 1:100-500.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); chemokine; SDF-1 alpha; CXC; CXC chemokine; CXC motif chemokine; CXCL; Cxcl12; H-SDF-1-alpha; interleukin-8 homolog; pre-B-cell growth-stimulating factor; SDF 1 a; SDF 1 alpha; SDF 1α; SDF-1 gamma; SDF1 alpha; SDF1α; stromal cell-derived factor-1 alpha; stromal cell-derived factor-1 gamma; thymic lymphoma cell-stimulating factor; unnamed protein product
Gene Aliases: Pbsf; Scyb12; Sdf1; Tlsf; Tpar1
UniProt ID: (Mouse) P40224
Entrez Gene ID: (Mouse) 20315, (Rat) 24772
patterning of blood vessels
ameboidal-type cell migration
neuron migration
positive regulation of endothelial cell proliferation
chemotaxis
defense response
immune response
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
germ cell development
axon guidance
brain development
motor neuron axon guidance
positive regulation of cell proliferation
negative regulation of cell proliferation
adult locomotory behavior
germ cell migration
T cell chemotaxis
telencephalon cell migration
regulation of cell migration
positive regulation of cell migration
organ regeneration
killing of cells of other organism
positive regulation of dopamine secretion
integrin activation
chemokine (C-X-C motif) ligand 12 signaling pathway
CXCL12-activated CXCR4 signaling pathway
T cell proliferation
positive regulation of neuron differentiation
positive regulation of cell adhesion
positive regulation of axon extension involved in axon guidance
positive regulation of chemotaxis
induction of positive chemotaxis
detection of temperature stimulus involved in sensory perception of pain
detection of mechanical stimulus involved in sensory perception of pain
positive regulation of protein kinase B signaling
regulation of calcium ion transport
cell chemotaxis
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
dopaminergic neuron differentiation
positive regulation of monocyte chemotaxis
positive regulation of calcium ion import
cell-cell adhesion
positive regulation of myoblast fusion
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of leukocyte tethering or rolling
positive regulation of vasculature development
cellular response to chemokine
positive regulation of T cell migration
negative regulation of dendritic cell apoptotic process
response to hypoxia
response to mechanical stimulus
cell migration
response to peptide hormone
response to ultrasound
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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