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Invitrogen
Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eBioscience™
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FIGURE: 1 / 3
Phospho-BTK/ITK (Tyr551, Tyr511) Antibody (14-9015-80) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 0.5 µg/mL of Anti-Human/Mouse Phospho-BTK/ITK (Y551/Y511) Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-9015-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
M4G3LN
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
25 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.
In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.
Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.
Applications Reported: This M4G3LN antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated M4G3LN is recommended for use in intracellular flow cytometry.).
Applications Tested: This M4G3LN antibody has been tested by immunoblotting of stimulated Daudi B cells. This can be used at less than or equal to 5 µg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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