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Invitrogen

CD284 (TLR4) Monoclonal Antibody (UT41), eBioscience™

22 Published Figures
18 References
View all (62) TLR4 (CD284) antibodies

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Product Details

14-9041-37

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test
View 13 publications 13 publications

Functional Assay (Functional)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

UT41

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

2 mg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865323

Product Specific Information

Description: The UT41 monoclonal antibody was generated against BaF3 cells overexpressing mouse TLR4. So far, at least twelve members of the Toll family have been identified. This family of type I transmembrane protein is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4, which is approximately 100 kDa, is expressed by myeloid lineage cells.

The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex. Please contact eBioscience Technical Support for further information.

Applications Reported: This UT41 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UT41 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: lipopolysaccharide response; TLR4; Tlr4; similar to Toll/IL-1 superfamily; toll homolog; Toll like receptor 4; unnamed protein product

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Gene Aliases: Lps; Ly87; Ran/M1; Rasl2-8

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UniProt ID: (Mouse) Q9QUK6

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Entrez Gene ID: (Mouse) 21898

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Function(s)
lipopolysaccharide binding lipopolysaccharide receptor activity transmembrane signaling receptor activity receptor binding protein binding signaling receptor activity identical protein binding phosphatidylinositol 3-kinase binding protein heterodimerization activity
Process(es)
microglial cell activation positive regulation of cytokine production activation of innate immune response toll-like receptor signaling pathway wound healing involved in inflammatory response immune effector process B cell proliferation involved in immune response nitric oxide production involved in inflammatory response regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway innate immune response-activating signal transduction growth plate cartilage morphogenesis leukotriene metabolic process phagocytosis inflammatory response immune response response to oxidative stress signal transduction I-kappaB kinase/NF-kappaB signaling activation of NF-kappaB-inducing kinase activity I-kappaB phosphorylation response to bacterium gene expression positive regulation of platelet activation positive regulation of gene expression astrocyte development microglia differentiation positive regulation of smooth muscle cell migration neurogenesis signal transduction involved in regulation of gene expression positive regulation of cell migration positive regulation of B cell proliferation lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide detection of lipopolysaccharide regulation of tumor necrosis factor production negative regulation of interferon-gamma production negative regulation of interleukin-17 production negative regulation of interleukin-23 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-13 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade toll-like receptor 4 signaling pathway regulation of toll-like receptor 4 signaling pathway TRIF-dependent toll-like receptor signaling pathway cellular response to platelet-derived growth factor stimulus macrophage activation defense response to bacterium positive regulation of macrophage activation positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response leukocyte activation positive regulation of MHC class II biosynthetic process positive regulation of nitric oxide biosynthetic process response to ethanol mast cell activation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation regulation of inflammatory response positive regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium detection of temperature stimulus involved in sensory perception of pain detection of mechanical stimulus involved in sensory perception of pain NK T cell activation detection of stimulus bone morphogenesis intestinal epithelial structure maintenance positive regulation of macrophage cytokine production ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway response to fatty acid cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to mechanical stimulus cellular response to interferon-gamma negative regulation of cold-induced thermogenesis cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of cytokine production involved in inflammatory response positive regulation of cytokine production involved in inflammatory response positive regulation of NLRP3 inflammasome complex assembly positive regulation of reactive oxygen species biosynthetic process positive regulation of cellular response to macrophage colony-stimulating factor stimulus positive regulation of matrix metallopeptidase secretion cellular response to beta-amyloid positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of extrinsic apoptotic signaling pathway
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