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Invitrogen

EZH2 Monoclonal Antibody (AC22), eBioscience™

1 Published Figure
1 Reference
View all (62) EZH2 antibodies

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Product Details

14-9867-37

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-Dependent
-

Immunohistochemistry (IHC)

Assay-Dependent
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Immunoprecipitation (IP)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

AC22

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

2 mg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865489

Product Specific Information

Description: The monoclonal antibody AC22 recognizes EZH2 (Enhancer of zeste homolog 2), a member of the polycomb group (PcG) proteins responsible for repression of gene expression through recognition and modification of histone methylation and chromatin structure. EZH2 comprises the catalytic subunit of the polycomb repressive complex 2 (PRC2) and directly represses gene expression through methylation of histone H3 on lysine 27 (H3K27) as well as direct protein targets such as GATA4. The PRC2 complex gene repression is necessary for proper development and differentiation. Underexpression of EZH2 is linked to abnormal developmental patterning and a loss of stem cell pluripotency, while overexpression of EZH2 is present in many tumors and correlates with poor prognosis in melanoma, breast, and prostate cancer.

Applications Reported: This AC22 antibody has been reported for use in immunoprecipitation, western blotting, and immunocytochemistry.

Applications Tested: This AC22 antibody has been tested by immunocytochemistry of methanol-fixed cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: an; enhancer of zeste homolog 2; enhancer of zeste homolog 2; transcription repressor; chromatin modifier; ENX 1; EZH 2; EZH-2; lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780; putative transcriptional regulator; unnamed protein product

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Gene Aliases: ENX-1; ENX1; Enx1h; EZH2b; KMT6; KMT6A; mKIAA4065; WVS; WVS2

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UniProt ID: (Human) Q15910, (Mouse) Q61188

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Entrez Gene ID: (Human) 2146, (Mouse) 14056, (Rat) 312299

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription corepressor binding DNA binding chromatin binding transcription corepressor activity RNA binding protein binding protein-lysine N-methyltransferase activity chromatin DNA binding nucleosome binding histone methyltransferase activity histone binding ribonucleoprotein complex binding sequence-specific DNA binding histone methyltransferase activity (H3-K27 specific) primary miRNA binding lncRNA binding histone H3 methyltransferase activity histone H3K27 trimethyltransferase activity promoter-specific chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter chromatin organization chromatin remodeling regulation of transcription, DNA-templated positive regulation of cell proliferation negative regulation of gene expression positive regulation of epithelial to mesenchymal transition hippocampus development B cell differentiation positive regulation of cell migration chromatin silencing by small RNA heterochromatin assembly telomeric heterochromatin assembly response to estradiol regulation of circadian rhythm positive regulation of MAP kinase activity positive regulation of GTPase activity negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated negative regulation of retinoic acid receptor signaling pathway cell development synaptic transmission, GABAergic positive regulation of protein serine/threonine kinase activity regulation of kidney development facultative heterochromatin formation positive regulation of dendrite development negative regulation of cytokine production involved in inflammatory response positive regulation of cell cycle G1/S phase transition regulation of protein phosphorylation methylation-dependent chromatin silencing regulation of transcription from RNA polymerase II promoter gene expression regulation of gene expression regulation of gliogenesis skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration cardiac muscle hypertrophy in response to stress cerebellar cortex development negative regulation of transcription elongation from RNA polymerase II promoter cellular response to trichostatin A hepatocyte homeostasis regulation of cell proliferation negative regulation of epidermal cell differentiation regulation of neurogenesis negative regulation of striated muscle cell differentiation cellular response to hydrogen peroxide G1 to G0 transition protein localization to chromatin immunoglobulin heavy chain V-D-J recombination liver regeneration constitutive heterochromatin formation response to tetrachloromethane negative regulation of G1/S transition of mitotic cell cycle
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