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Invitrogen
Vimentin Monoclonal Antibody (V9), eBioscience™
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FIGURE: 1 / 20
Vimentin Antibody (14-9897-82) in ICC/IF
Immunofluorescence analysis of Vimentin was performed using 70 confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Vimentin Monoclonal Antibody (V9), eBioscience™ (Product # 14-9897-80) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More
Product Details
14-9897-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Human,
Rat
Published species
Cynomolgus monkey,
Dog,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
V9
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.
This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.
Applications Reported: This V9 antibody has been reported for use in western blotting, immunocytochemistry, and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P).
Applications Tested: This V9 antibody has been tested by immunocytochemistry on paraformaldehyde fixed and permeabilized SK-N-SH or C6 cell lines and by western blot on cell lysates prepared from HeLa cells. This antibody can be used at less than or equal to 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; FLJ36605; Hel113; RP11-124N14.1; unnamed protein product; VIM; VIME; Vimentin; vimentin C-terminus
Gene Aliases: VIM
UniProt ID: (Human) P08670, (Dog) A0A8C0N8E3, (Rat) P31000
Entrez Gene ID: (Chicken) 420519, (Human) 7431, (Dog) 477991, (Rat) 81818
RNA binding
double-stranded RNA binding
structural constituent of cytoskeleton
structural constituent of eye lens
protein binding
protein domain specific binding
identical protein binding
binding, bridging
scaffold protein binding
keratin filament binding
glycoprotein binding
structural molecule activity
protein C-terminus binding
kinase binding
protein kinase binding
protein phosphatase 2A binding
positive regulation of gene expression
negative regulation of neuron projection development
astrocyte development
neuron projection development
positive regulation of collagen biosynthetic process
regulation of mRNA stability
intermediate filament-based process
intermediate filament organization
Bergmann glial cell differentiation
lens fiber cell development
cellular response to lipopolysaccharide
cellular response to muramyl dipeptide
cellular response to interferon-gamma
aging
response to radiation
response to zinc ion
response to selenium ion
response to estradiol
response to vitamin D
skeletal muscle tissue regeneration
cellular response to fibroblast growth factor stimulus
fat cell differentiation
decidualization
regulation of axonogenesis
positive regulation of glial cell proliferation
SMAD protein signal transduction
regulation of Schwann cell migration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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