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HBSS, calcium, magnesium, no phenol red, 1000 mL - FAQs

HBSS, calcium, magnesium, no phenol red, 1000 mL - FAQs

View additional product information for HBSS, calcium, magnesium, no phenol red - FAQs (14025076, 14025092, 14025126, 14025134)

7 product FAQs found

What is the pH of HBSS, calcium, magnesium, no phenol red at room temperature.

At room temperature, HBSS, calcium, magnesium, no phenol red has a pH of 6.7 - 7.8.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to add additional HEPES to Hank's Balanced Salt Solution (HBSS) (Cat. No. 14025xxx)?

HBSS (Cat. No. 14025xxx) does not contain HEPES and it needs to be externally supplemented. The complete formulation is available here (https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.153.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why do you offer HBSS with and without phenol red?

Phenol red is a pH indicator which allows you to determine the optimal atmospheric conditions for your cell line during your particular assays. We offer both versions to allow you the option to utilize this indicator in your media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use HBSS (with calcium and magnesium) to wash my cells before dissociation?

No, a saline solution that contains calcium and magnesium should not be used to wash cells before dissociation.

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Is a precipitate in HBSS (with calcium and magnesium) normal?

No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.