HBSS, calcium, magnesium, no phenol red
HBSS, calcium, magnesium, no phenol red
Gibco™

HBSS, calcium, magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,자세히 알아보기
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카탈로그 번호수량
140250761000 mL
14025092500 mL
1402513410 x 500 mL
140251266 x 1000 mL
카탈로그 번호 14025076
제품 가격(KRW)
71,000
온라인 행사
Ends: 31-Dec-2025
74,000
할인액 3,000 (4%)
Each
카트에 추가하기
수량:
1000 mL
Customize this product
제품 가격(KRW)
71,000
온라인 행사
Ends: 31-Dec-2025
74,000
할인액 3,000 (4%)
Each
카트에 추가하기
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation.

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.

This HBSS is modified as follows:

WithWithout
• Calcium• Phenol Red
• Magnesium• Sodium Pyruvate
• Glucose

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco™ HBSS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ HBSS product made in our Scotland facility (14065-049). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

This product is not used for in vitro diagnostic purpose in some countries.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
화학물질 이름 또는 재질Hanks' Balanced Salt Solution (HBSS)
포함No Sodium Pyruvate
제조 품질cGMP-compliant under the ISO 13485 standard
삼투압270 - 305 mOsm/kg
권장 스토리지Storage conditions: 15–30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
멸균Sterile-filtered
농도1 X
용도(애플리케이션)Mammalian Cell Culture
물리적 형태Liquid
제품라인Gibco
수량1000 mL
용액 유형Hank's Balanced Salt
pH6.7 - 7.8
Unit SizeEach

자주 묻는 질문(FAQ)

What is the pH of HBSS, calcium, magnesium, no phenol red at room temperature.

At room temperature, HBSS, calcium, magnesium, no phenol red has a pH of 6.7 - 7.8.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to add additional HEPES to Hank's Balanced Salt Solution (HBSS) (Cat. No. 14025xxx)?

HBSS (Cat. No. 14025xxx) does not contain HEPES and it needs to be externally supplemented. The complete formulation is available here (https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.153.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why do you offer HBSS with and without phenol red?

Phenol red is a pH indicator which allows you to determine the optimal atmospheric conditions for your cell line during your particular assays. We offer both versions to allow you the option to utilize this indicator in your media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use HBSS (with calcium and magnesium) to wash my cells before dissociation?

No, a saline solution that contains calcium and magnesium should not be used to wash cells before dissociation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Multicolor and electron microscopic imaging of connexin trafficking.
Authors: Gaietta Guido; Deerinck Thomas J; Adams Stephen R; Bouwer James; Tour Oded; Laird Dale W; Sosinsky Gina E; Tsien Roger Y; Ellisman Mark H;
Journal:Science
PubMed ID:11964472
'Recombinant proteins containing tetracysteine tags can be successively labeled in living cells with different colors of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy. Here we used this approach to show that newly synthesized connexin43 was transported predominantly in ... More