SuperSignal™ West HisProbe Kit
SuperSignal™ West HisProbe Kit
Thermo Scientific™

SuperSignal™ West HisProbe Kit

The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged proteinRead more
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Catalog number 15168
Price (USD)/ Each
658.00
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Price (USD)/ Each
658.00
Add to cart
SuperSignal™ West HisProbe Kit
Catalog number15168
Price (USD)/ Each
658.00
-
Add to cart
The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged protein by western blotting. HisProbe-HRP Conjugate is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.

Features of the SuperSignal West HisProbe Kit:

Fast—His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
Economical—no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
High specificity—HisProbe-HRP can recognize his-tags independent of adjacent tags.
Less steric hindrance—HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC). The Thermo Scientific SuperSignal West HisProbe Kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate.

Related Products
HisProbe™-HRP Conjugate
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce SuperSignal West HisProbe Kit
Product TypeHisProbe Detection Kit
Detection MethodChemiluminescence
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LineHisProbe™, SuperSignal™
Quantity685 mL
ConjugateHRP
SubstrateHorseradish Peroxidase
ReactivityAll species
Sufficient For62 mini blots or 5000 sq cm membrane
Unit SizeEach
Contents & Storage
Sufficient For: 62 mini blots or 5000 cm2 membrane
• HisProbe-HRP Conjugate, 2 mg
• SuperSignal West Pico Luminol/Enhancer Solution, 250 mL
• SuperSignal West Pico Stable Peroxide Solution, 250 mL
• Blocker BSA in TBS (10X), 125 mL
• TBS Packs (each makes 500 mL), 10 packs
• Surfact-Amps 20 Detergent Solution, 6 x 10 mL

Store HisProbe-HRP, Blocker BSA in TBS and Surfact-Amps 20 at 4°C. Store remaining components at 4°C.