Thermo Scientific™

HisProbe™-HRP Conjugate

Catalog number: 15165
Thermo Scientific™

HisProbe™-HRP Conjugate

Catalog number: 15165
Catalog Number
15165
Unit Size
2 mg
Price (USD)
272.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
151652 mg
272.00
Your Price:
***
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Thermo Scientific HisProbe-HRP is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.

Features of HisProbe-HRP Conjugate:

Fast—His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
Economical—no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
High specificity—HisProbe-HRP can recognize his-tags independent of adjacent tags.
Less steric hindrance—HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC). The Thermo Scientific SuperSignal West HisProbe Kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate.

Related Products
SuperSignal™ West HisProbe Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
Pierce HisProbe-HRP Conjugate
Detection Method
Western Blot
Form
Solid
Product Line
HisProbe™
Product Type
HRP Conjugate
Quantity
2 mg
Reactivity
All species

Contents & Storage

Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.

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