Citations et références (5)

Gibco™

Amphotéricine B

Name: Amphotéricine B
SKU: 15290026
Price: 51.75 EUR

L’amphotéricine B est la version générique de Fungizone. “Fungizone” est une marque commerciale d’E.R. Squibb & Sons, LLC. L’amphotéricine BAfficher plus

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Référence	Quantité
15290026	50 ml
15290018	20 ml

2 options

Référence 15290026

Prix (EUR)

51,75

Each

Quantité:

50 ml

Prix (EUR)

51,75

Each

L’amphotéricine B est la version générique de Fungizone. “Fungizone” est une marque commerciale d’E.R. Squibb & Sons, LLC. L’amphotéricine B est un antifongique produit par Streptomyces nodosus. Elle empêche la croissance de champignons en causant une perméabilité accrue de la membrane plasmique fongique. Elle se lie activement aux stérols et entraîne la formation de pores. Elle sert à empêcher la contamination des cultures cellulaires par la levure et les champignons multicellulaires. L’amphotéricine B Gibco contient 250 µg d’amphotéricine B et 205 µg de désoxycholate de sodium par ml d’eau distillée. Les plages de concentration de travail recommandées s’échelonnent entre 0,25 et 2,50 µg / ml.

Fabrication conforme aux BPFa sur deux sites
Pour assurer la continuité de la chaîne d’approvisionnement, nous fabriquons l’amphotéricine B Gibco dans deux sites distincts, situés à Grand Island, dans l’État de New York, et en Écosse, au Royaume-Uni. Les deux sites sont conformes aux exigences BPFa de fabrication, certifiés ISO 13485 et homologués par la FDA comme fabricants de dispositifs médicaux.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Concentration0,25 à 2,5 μg/ml

À utiliser avec (application)Prévention de la contamination par culture cellulaire

Gamme de produitsFungizone

Quantité50 ml

Durée de conservation12 mois

Conditions d’expéditionGlace carbonique

FormeLiquide

Type de produitAntifongique

StérilitéStérile

Unit SizeEach

Contenu et stockage

Conditions de stockage : -5 à -20°C
Conditions d’expédition : Congelés sur glace sèche
Durée de conservation : 12 mois à compter de la date de fabrication

Foire aux questions (FAQ)

How many freeze/thaw cycles do you recommend for Amphotericin B?

Amphotericin B can be freeze-thawed 2-3 times without appreciable loss of potency.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the stability of Amphotericin B when stored at 4 degrees C?

Amphotericin B in solution is stable at 2-8 degrees C for approximately 4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Fungizone and Amphotericin B?

Amphotericin B is the generic version of Fungizone. Fungizone is a trademark of E.R. Squibb & Sons, LLC.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (5)

Citations et références

Abstract

PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions.

Authors:Wiatrak B, Kubis-Kubiak A, Piwowar A, Barg E

Journal:Cells

PubMed ID:32295099

'The PC12 cell line is one of the most commonly used in neuroscience research, including studies on neurotoxicity, neuroprotection, neurosecretion, neuroinflammation, and synaptogenesis. Two types of this line are available in the ATCC collection: traditional PC12 cells grown in suspension and well-attached adherent phenotype. PC12 cells grown in suspension tend ... More

Generation of human brain region-specific organoids using a miniaturized spinning bioreactor.

Authors:Qian X, Jacob F, Song MM, Nguyen HN, Song H, Ming GL

Journal:Nat Protoc

PubMed ID:29470464

Human brain organoids, 3D self-assembled neural tissues derived from pluripotent stem cells, are important tools for studying human brain development and related disorders. Suspension cultures maintained by spinning bioreactors allow for the growth of large organoids despite the lack of vasculature, but commercially available spinning bioreactors are bulky in size ... More

Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides.

Authors:Arrigo AP

Journal:Methods Mol Biol

PubMed ID:29177658

Human HspB1 (also denoted as Hsp27) belongs to the family of small (or stress) proteins (sHsps). The family, which contains ten members including aA,B-crystallin polypeptides, is characterized by a conserved C-terminal a-crystallin domain and molecular weights ranging from 20 to 40 kDa. Here, procedures are described for analyzing the dynamic oligomerization ... More

A protective Zika virus E-dimer-based subunit vaccine engineered to abrogate antibody-dependent enhancement of dengue infection.

Authors:Slon-Campos JL, Dejnirattisai W, Jagger BW, López-Camacho C, Wongwiwat W, Durnell LA, Winkler ES, Chen RE, Reyes-Sandoval A, Rey FA, Diamond MS, Mongkolsapaya J, Screaton GR

Journal:Nat Immunol

PubMed ID:31477918

Infections with dengue virus (DENV) and Zika virus (ZIKV) can induce cross-reactive antibody responses. Two immunodominant epitopes-one to precursor membrane protein and one to the fusion loop epitope on envelope (E) protein-are recognized by cross-reactive antibodies ... More

Establishment and Genetic Landscape of Precancer Cell Model Systems from the Head and Neck Mucosal Lining.

Authors:de Boer DV, Brink A, Buijze M, Stigter-van Walsum M, Hunter KD, Ylstra B, Bloemena E, Leemans CR, Brakenhoff RH

Journal:Mol Cancer Res

PubMed ID:30224542

Head and neck squamous cell carcinomas (HNSCC) develop in fields of genetically altered cells. These fields are often dysplastic and a subset can be recognized as (erythro)leukoplakia, but most are macroscopically invisible. There is a lack of adequate treatment options to eradicate these fields, whereas they underlie the development of ... More