PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions.
AuthorsWiatrak B, Kubis-Kubiak A, Piwowar A, Barg E
JournalCells
PubMed ID32295099
'The PC12 cell line is one of the most commonly used in neuroscience research, including studies on neurotoxicity, neuroprotection, neurosecretion, neuroinflammation, and synaptogenesis. Two types of this line are available in the ATCC collection: traditional PC12 cells grown in suspension and well-attached adherent phenotype. PC12 cells grown in suspension tend ... More
Generation of human brain region-specific organoids using a miniaturized spinning bioreactor.
AuthorsQian X, Jacob F, Song MM, Nguyen HN, Song H, Ming GL
JournalNat Protoc
PubMed ID29470464
Human brain organoids, 3D self-assembled neural tissues derived from pluripotent stem cells, are important tools for studying human brain development and related disorders. Suspension cultures maintained by spinning bioreactors allow for the growth of large organoids despite the lack of vasculature, but commercially available spinning bioreactors are bulky in size ... More
Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides.
AuthorsArrigo AP
JournalMethods Mol Biol
PubMed ID29177658
Human HspB1 (also denoted as Hsp27) belongs to the family of small (or stress) proteins (sHsps). The family, which contains ten members including aA,B-crystallin polypeptides, is characterized by a conserved C-terminal a-crystallin domain and molecular weights ranging from 20 to 40 kDa. Here, procedures are described for analyzing the dynamic oligomerization ... More
A protective Zika virus E-dimer-based subunit vaccine engineered to abrogate antibody-dependent enhancement of dengue infection.
AuthorsSlon-Campos JL, Dejnirattisai W, Jagger BW, López-Camacho C, Wongwiwat W, Durnell LA, Winkler ES, Chen RE, Reyes-Sandoval A, Rey FA, Diamond MS, Mongkolsapaya J, Screaton GR
JournalNat Immunol
PubMed ID31477918
Infections with dengue virus (DENV) and Zika virus (ZIKV) can induce cross-reactive antibody responses. Two immunodominant epitopes-one to precursor membrane protein and one to the fusion loop epitope on envelope (E) protein-are recognized by cross-reactive antibodies ... More
Establishment and Genetic Landscape of Precancer Cell Model Systems from the Head and Neck Mucosal Lining.
Authorsde Boer DV, Brink A, Buijze M, Stigter-van Walsum M, Hunter KD, Ylstra B, Bloemena E, Leemans CR, Brakenhoff RH
JournalMol Cancer Res
PubMed ID30224542
Head and neck squamous cell carcinomas (HNSCC) develop in fields of genetically altered cells. These fields are often dysplastic and a subset can be recognized as (erythro)leukoplakia, but most are macroscopically invisible. There is a lack of adequate treatment options to eradicate these fields, whereas they underlie the development of ... More